LOCUS       sequence-93450-e         799 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 590, Emission:
            649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Wang L, Jackson WC, Steinbach PA, Tsien RY
  TITLE     Evolution of new nonantibody proteins via iterative somatic 
            hypermutation.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Nov 30;101(48):16745-9. Epub 2004 Nov
            19.
  PUBMED    15556995
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2004-11-30"; volume: "101(48)"; pages: 
            "16745-9. Epub 2004 Nov 19"
COMMENT     Sequence Label: mPlum-pBAD
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..700)
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     complement(82..105)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(280..299)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(707..727)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(740..772)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(776..793)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 acagccaagc ttcgaattct taggcgccgg tggagtggcg gccctcggcg cgctcgtact
       61 gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc cagcttgatg tcggtcttgt
      121 aggcgccggg cagctgcacg ggcttcttgg ccatgtaggt ggtcttgacc tcggcgtcgt
      181 agtggccgcc gtccttcagc ctcagcctca tcttcatctc gcccttcagg gcgccgtcct
      241 cggggtacat ccgctcggag gaggcctccc agcccatggt cttcttctgc attacggggc
      301 cgtcggaggg gaagttggtg ccgcgcacct tcaccttgta gatgaactcg ccgtcctgca
      361 gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc
      421 acttgaagcc ctcggggaag gacagcttca agtagtcggg gatgtcggcg gggtgcttca
      481 cgtaggcctt ggagccgtac atgatctgag gggacaggat gtcccaggcg aagggcaggg
      541 ggccaccctt ggtcaccttc agcctggcgg tctgggtgcc ctcgtagggg cggccctcgc
      601 cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc tccttgaagc
      661 gcatgaactc cttgatgacc tcctcgccct tgctcaccat gctcgagccc tggaagtaca
      721 ggttctcgta cagatcccga cccatttgct gtccaccagt catgctagcc ataccatgat
      781 gatgatgatg atgagaacc
//
