LOCUS       sequence-93453-e         798 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 505, Emission:
            515. This plasmid encodes Clover H231L based on reference from Bajar
            et. al. Sci Rep. 2016..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-pBAD
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             35..57
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             77..94
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             98..130
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             143..163
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(215..236)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttctccatac ccgttttttt gggctagaaa taattttgtt taactttaag aaggagatat
       61 acatatgcgg ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca
      121 gcaaatgggt cgggatctgt acgagaacct gtacttccag ggctcgagca tggtgagcaa
      181 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      241 cggccacaag ttcagcgtcc gcggcgaggg cgagggcgat gccaccaacg gcaagctgac
      301 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      361 cttcggctac ggcgtggcct gcttcagccg ctaccccgac cacatgaagc agcacgactt
      421 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatctctt tcaaggacga
      481 cggtacctac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      541 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      601 caacttcaac agccacaacg tctatatcac ggccgacaag cagaagaacg gcatcaaggc
      661 taacttcaag atccgccaca acgttgagga cggcagcgtg cagctcgccg accactacca
      721 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcca
      781 tcagtccgcc ctgagcaa
//
