LOCUS       sequence-93562-e        1118 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral GFP reporter for testing enhancer activity in different 
            cell types. Insert is an enhancer identified in the intergenic 
            region of the keratin type II cluster..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1118)
  TITLE     Yu Lab-keratin enhancer GFP reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-Enh eGFP Reporter-muKC2-IGR8
FEATURES             Location/Qualifiers
     source          1..1118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(697..796)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(813..832)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(814..832)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(837..853)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(837..853)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tgantttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
       61 ttataatgcc aactttgtac aaaaaagcag gctttgaaga aatgtttcct tcctacatcg
      121 gacagaagca aagacaagtg tccaccagga ggtgacaact gaaagccagt cccacactcc
      181 cactgcagcc ctgtggcaca cacagctctc gctttggctt ctgcccttgg tgaccctgct
      241 tcctttggcc acctgcccga gcctggctct gaggcgtaag ccgggtgctg tcaggcatgg
      301 aacgggagtg ggagggtgtg ttccctcaga gctttctggg tgcaggaggc gaagccttcc
      361 cattcaacta gggattacgg atgctgaggc ctctatcacc gtcagcccta acctggggaa
      421 cagtgtggga aaatgtcttg ctctccctgg tcagatgggt gcccttgccc cactgggtca
      481 cctaacccca attgccccat ggcctgagtc actctgaggg aaccagagcc aaccagagat
      541 gctcaggaga cccagggagc tgggaggaag ggaaggccag aaaagtccat ggatcagtgg
      601 ctcgttctcc tgcccctggc atgtgccagc agggactttg aacacttccc accaccaaag
      661 aacactcgat gaagctttca ggcaacagag atagaaaccc agctttcttg tacaaagttg
      721 gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
      781 ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca
      841 tagctgtttc ctggnagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
      901 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacanggg
      961 gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg
     1021 ctgatttata tgggtatnaa tgggctcgcn atnatgtcgg gnaatcnnng cgacnatcta
     1081 tcgcttgnnn gggaancccg atnnnncnnn annnnntt
//
