LOCUS       sequence-93696-e         850 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses Opto-hRET (light-activated receptor tyrosine kinase) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Grusch M, Schelch K, Riedler R, Reichhart E, Differ C, Berger W, 
            Ingles-Prieto A, Janovjak H
  TITLE     Spatio-temporally precise activation of engineered receptor tyrosine
            kinases by light.
  JOURNAL   EMBO J. 2014 Jul 1. pii: e201387695.
  PUBMED    24986882
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2014 Jul 1. pii: e201387695."
COMMENT     Sequence Label: Opto-hRET_317
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      108..117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             114..155
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagaca
       61 caagctggct agcgtttaaa cgggccctct agactcgagc ggccgcggcc accatgggga
      121 gtagcaagag caagcctaag gaccccagcc agcgcctcga cgtcaccggt cactgctacc
      181 acaagtttgc ccacaagcca cccatctcct cagctgagat gaccttccgg aggcccgccc
      241 aggccttccc ggtcagctac tcctcttcca gtgcccgccg gccctcgctg gactccatgg
      301 agaaccaggt ctccgtggat gccttcaaga tcctggagga tccaaagtgg gaattccctc
      361 ggaagaactt ggttcttgga aaaactctag gagaaggcga atttggaaaa gtggtcaagg
      421 caacggcctt ccatctgaaa ggcagagcag ggtacaccac ggtggccgtg aagatgctga
      481 aagagaacgc ctccccgagt gagcttcgag acctgctgtc agagttcaac gtcctgaagc
      541 aggtcaacca cccacatgtc atcaaattgt atggggcctg cagccaggat ggcccgctcc
      601 tcctcatcgt ggagtacgcc aaatacggct ccctgcgggg cttcctccgc gagagccgca
      661 aagtggggcc tggctacctg ggcagtggag gcagccgcaa ctccagctcc ctggaccacc
      721 cggatgagcg ggccctcacc atgggcgacc tcatctcatt tgcctggcag atctcacagg
      781 ggatgcagta tctggccgag atgaagctcg ttcatcggga cttggcagcc agaaacatcc
      841 tggtagctga
//
