LOCUS       sequence-93917-e        1017 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: pmTagBFP2-C1
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    142..207
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(208..918)
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     regulatory      915..924
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(957..1008)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(986..1006)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
       61 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
      121 gatcagttat ctagatccgg tggatcccgg gcccgcggta ccgtcgactg cagaattcga
      181 agcttgagct cgagatctga gtccggaatt aagcttgtgc cccagtttgc tagggaggtc
      241 gcagtatctg gccactgcca cctcgtgctg ctcgacgtag gtctcgttgt tggcctcctt
      301 gattctttcc agtctgtagt ccacatagta gacgccaggc atcttgaggt tcttagcggg
      361 tttcttggat ctatatgtgg tcttggcgtt tgcgatcaga tggctcccgc ccacgagctt
      421 cagggccatg tcgtttctgc cttccaggcc gccgtcagcg gggtacagcg tctcggtgaa
      481 ggcctcccag ccgagtgttt tcttctgcat cacagggccg ttggatgtga agttcacccc
      541 tctgatcttg acgttgtaga tgaggcagcc gtcctggagg ctggtgtcct gggtagcggt
      601 cagcacgccc ccgtcttcgt atgtggtgac tctctcccat gtgaagccct cagggaagga
      661 ctgcttgaag aagtcgggga tgccctgggt gtggttgatg aaggtcttgc tgccgtagag
      721 gaagctagta gccaggatgt cgaaggcgaa ggggagaggg ccgccctcga ccaccttgat
      781 tctcatggtc tgggtgccct cgtagggctt gccttcgccc tcggatgtgc acttgaagtg
      841 atggttgtcc acggtgccct ccatgtacag cttcatgtgc atgttctcct taatcagctc
      901 ttcgccctta gacaccatgg tggccaccgc tagcggatct gacggttcac taaaccagct
      961 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcg
//
