LOCUS       sequence-93947-e         756 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  silence doxycyclin inducible pUltra-dox.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  TITLE     pUltra plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUltra-puro-RTTA3
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(36..69)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(85..195)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgag tcgacataac ttcgtatagt acacattata
       61 cgaagttatg aattcttacc cagggagcat gtcaaggtca aaatcgtcaa gagcgtcagc
      121 aggcagcata tcaaggtcaa agtcgtcaag ggcatcggct gggagcatgt ctaagtcaaa
      181 atcgtcaagg gcgtcggtcg gcccgccgct ttcgcacttt agctgtttct ccaggccaca
      241 tatgattagt tccaggccga aaaggaaggc aggttcggct ccctgccggt cgaacagctc
      301 aattgcttgt ctcagaagtg ggggcataga atcggtggta ggtgtctctc tttcctcttt
      361 tgctacttga tgctcctgtt cctccaatac gcagcccagt gtaaagtggc ccacggcgga
      421 cagagcgtac agtgcgttct ccagggagaa gccttgctga cacaggaacg cgagctgatt
      481 ttccagggtt tcgtactgtt tctctgttgg gcgggtgccg agatgcactt tagccccgtc
      541 gcgatgtgag aggagagcac agcggtatga cttggcgttg ttccgcagaa agtcttgcca
      601 tgactcgcct tccagggggc agaagtgggt atgatgcctg tccagcatct cgattggcag
      661 ggcatcgagc agggcccgct tgttcttcac gtgccagtac agggtaggct gctcaactcc
      721 cagcttttga gcgagtttcc ttgtcgtcag gccttc
//
