LOCUS       sequence-94109-e         857 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses full-length Nephrin precursor ectodomain in mammalian 
            cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF, Wright 
            GJ
  TITLE     A human platelet receptor protein microarray identifies 
            FcepsilonR1alpha as an activating PEAR1 ligand.
  JOURNAL   Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946.
  PUBMED    25713122
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2015 Feb 23. pii: mcp.M114.046946."
COMMENT     Sequence Label: NPHS-bio-His
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(69..86)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aataaatttc ctttattagc cagaggtcga ggtcggggga tccttaatta atcacgtgtc
       61 tagactcaat ggtggtgatg gtggtggggg cccagagggg ccagaggagg caggttcctg
      121 tccctgtggt tccacagcat cttctgggcg tccaggatgt ggtgcaggct gccggaattc
      181 aaccctttgg ataaaacctg gatcttggag tccatcttga cctcttcacc ttcactcagt
      241 agacattgcc acacccctgc ttcaggggcc tgcacttgaa tcactttctc ctgcctggag
      301 accctggcct cctgattctc ctgcttcaag atcagtctca tcttgggtga ggtgggtccc
      361 atcacctcac aggtcaaagt gttgctgtcg ggctgagtca ctttcatcac caccaggttc
      421 acttcctgat acagtatccc tctgtccaga gtcagggtca ggttgccaga accagcaaac
      481 tgaagggaga cctggggtat ctgaagggtg agtgggagcg tttcggacag ctggaacttg
      541 gggttgctag tagacttctg cacagacacc ttttggttct ttagggagaa ggtgatccag
      601 gactgggaag aaggagcctt ctctgccttc catctcaact ctccctgcag gctttcctct
      661 ccaaggttga gtgggaagga gaactccgct gactccccct cactcttata ggccgtgatg
      721 gaggtcgacg gcgcgccagg cagcagaggc aggccagaag ggccgctagg aggctcggta
      781 ggcagctggt cctctggctc gccgctaggc tggtgcaggc caggtgtggt gatgggcagc
      841 tgggtgccct tatcggc
//
