LOCUS       sequence-94194-e        1037 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  AAV-mediated expression of ChR2-mCherry under the GFAP promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Perea G, Yang A, Boyden ES, Sur M
  TITLE     Optogenetic astrocyte activation modulates response selectivity of 
            visual cortex neurons in vivo.
  JOURNAL   Nat Commun. 2014;5:3262. doi: 10.1038/ncomms4262.
  PUBMED    24500276
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014"; volume: "5:3262. doi"; pages: " 
            10.1038/ncomms4262"
COMMENT     Sequence Label: pAAV-GFAP104-ChR2-mCherry
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..770)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(341..360)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     610..628
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt tgtacagaat
       61 tcttacttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc gcgttcgtac
      121 tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat gttgacgttg
      181 taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac ctcagcgtcg
      241 tagtggccgc cgtccttcag cttcagcctc tgcttgatct cgcccttcag ggcgccgtcc
      301 tcggggtaca tccgctcgga ggaggcctcc cagcccatgg tcttcttctg cattacgggg
      361 ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc gccgtcctgc
      421 agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc
      481 cacttgaagc cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc ggggtgcttc
      541 acgtaggcct tggagccgta catgaactga ggggacagga tgtcccaggc gaagggcagg
      601 gggccaccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg gcggccctcg
      661 ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg caccttgaag
      721 cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac taccggtgcc
      781 gctggcacgg ctccggcctc ggcttcgtct tcgacgagag tctcgacctc gatctccgtt
      841 ccgccgatgt tcagtttggt ggttttgcgg atatctccgt gaatcaatat gtgctcgtgg
      901 atcaggacgc gcaggtagtg tcccaacaac ccccaacaat ttttactcat cagatcaata
      961 atcgtgtgac ctacggtgga gccatagacg ctcaggacgc caaaaccttc gggccccaaa
     1021 atgaagagaa ttgggaa
//
