LOCUS       sequence-94345-e         867 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses GFP-Tagged CERT PH domain in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Levine TP, Munro S
  TITLE     Targeting of Golgi-specific pleckstrin homology domains involves 
            both PtdIns 4-kinase-dependent and -independent components.
  JOURNAL   Curr Biol. 2002 Apr 30;12(9):695-704.
  PUBMED    12007412
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2002-04-30"; volume: "12(9)"; pages: "695-704"
COMMENT     Sequence Label: pRS406-PHO5-GFP-CERT PH domain
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(15..33)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        101..205
                     /gene="bla"
                     /label=AmpR promoter
     CDS             390..768
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(424..443)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgcgcgagag aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
       61 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      121 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      181 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      241 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      301 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
      361 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
      421 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
      481 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
      541 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
      601 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
      661 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
      721 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
      781 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
      841 taaagttgca ggaccacttc tgcgctc
//
