LOCUS       sequence-94347-e         760 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses GFP-Tagged Scs2 in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Loewen CJ, Roy A, Levine TP
  TITLE     A conserved ER targeting motif in three families of lipid binding 
            proteins and in Opi1p binds VAP.
  JOURNAL   EMBO J. 2003 May 1;22(9):2025-35.
  PUBMED    12727870
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2003-05-1"; volume: "22(9)"; pages: "2025-35"
COMMENT     Sequence Label: pRS406-PHO5-GFP-Scs2
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(3..21)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        89..193
                     /gene="bla"
                     /label=AmpR promoter
     CDS             378..756
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(412..431)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
       61 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      121 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      181 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      241 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
      301 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
      361 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
      421 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
      481 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
      541 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
      601 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
      661 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
      721 tgacaccacg atgcctgtag caatggcaac aacgttgcgc
//
