LOCUS       sequence-94720-e         812 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: ATP5O_pCSdest
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             261..272
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatggctgc cccagcagtg tccgggctct
      181 cccggcaggt gcgatgcttc agtacctctg tggtcagacc atttgccaag cttgtgaggc
      241 ctcctgttca ggtatacggt attgaaggtc gctatgccac agctctttat tctgctgcat
      301 caaaacagaa taagctggag caagtagaaa aggagttgtt gagagtagca caaatcctga
      361 aggaacccaa agtggctgct tctgttttga atccctatgt gaagcgttcc attaaagtga
      421 aaagcctaaa tgacatcaca gcaaaagaga ggttctctcc cctcactacc aacctgatca
      481 atttgcttgc tgaaaatggt cgattaagca atacccaagg agtcgtttct gccttttcta
      541 ccatgatgag tgtccatcgc ggagaggtac cttgcacagt gacctctgca tctcctttag
      601 aagaagccac actctctgaa ttaaaaactg tcctcaagag cttcctaagt caaggccaag
      661 tattgaaatt ggaggctaag actgatccgt caatcttggg tggaatgatt gtgcgcattg
      721 gcgagaaata tgttgacatg tctgtcaaga ccaagattca gaagctgggc agggctatgc
      781 gggagattgt ctagaaccca gctttcttgt ac
//
