LOCUS       sequence-95640-e        1012 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  retroviral pBabe puro vector encoding N-terminally FLAG tagged 
            mutant human IRE1a deleted from amino acids P28 to D408 and bearing 
            mutation K599A (kinase domain mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Volmer R, van der Ploeg K, Ron D
  TITLE     Membrane lipid saturation activates endoplasmic reticulum unfolded 
            protein response transducers through their transmembrane domains.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):4628-33. doi: 
            10.1073/pnas.1217611110. Epub 2013 Mar 4.
  PUBMED    23487760
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1217611110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-19- 19"; volume: "110"; issue: "12"; pages: "4628-33"
COMMENT     Sequence Label: Flag_HsIRE1a_deltaP29_D408_K599A_pBabePuro
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggccgccagt gtgatggata tctgcagaat tccgggctgg ctcctgctgc ctctgcagaa
       61 acactagttg tgaccaggcc ctcagtcaca gctggggcca ccagaacaga ggggccgccc
      121 tcgctcagag ggcgtctgga gtcactgggg gctggggctc tgggggctcg tggaagtagt
      181 agggctggaa gagtctctcg tggctgcaca gctccatggc ccggtaggtg tgtgcgagga
      241 ggtgggggaa gcgagacgtg aagtagcaca cgaagtcgtc ggggagggac cccagcgtct
      301 cccgcacctc tgcaggcagc tcccggtagt ggtgcttctt atttctcatg gctcggagga
      361 gatctctgac agaaccacct ttataggtcc tgaatttacg caggtctgtc tggaggggga
      421 cagtgatgtt ctcccgccag tccatcttca ccacggctct cccgcctctc tctaactgct
      481 tcacgatcgg gccatccagg gattcctttt ctattctgtc gctcacgtcc tggaagaact
      541 ggagctgctt ctctaggctc cagaagaacg ggtgtttgag cacgtgcttc gctgaggggc
      601 gtttctgagg atccatcgca atcatcttct ctatcaattc acgtgcaatg acgtcttcgt
      661 gcttctctgg gtgcaagcag tcaaggctgc aggcacccag gaggatgttg gcctgccgct
      721 gcagggactt gccaaaaggg tggctgccct cagagattac gtagtaaaag acgcagcctg
      781 cagaaaagat gtccaccgtg taggtagggt tctccttaca gtcttcgctc agcatctctg
      841 gagcgatcca gccttctgtg ccaggcaccc cagatcggcg gctgaaactg tgtctgccca
      901 ctgccagctt cttgcagagg ccaaagtcgg agatcatggc cttgatcttg ccgtgtgcat
      961 tgggcatgga tatgaggatg ttgtgtggct ttaggtctct gtgaacgatg tt
//
