LOCUS       sequence-95769-e         953 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expressed murine IFITM3-C72A with an N-terminal HA tag in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Yount JS, Moltedo B, Yang YY, Charron G, Moran TM, Lopez CB, Hang HC
  TITLE     Palmitoylome profiling reveals S-palmitoylation-dependent antiviral 
            activity of IFITM3.
  JOURNAL   Nat Chem Biol. 2010 Aug;6(8):610-4. doi: 10.1038/nchembio.405. Epub 
            2010 Jul 4.
  PUBMED    20601941
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.405"; journalName: "Nat Chem Biol"; date: 
            "2010-08"; volume: "6"; issue: "8"; pages: "610-4"
COMMENT     Sequence Label: pCMV-HA-mIFITM3-C72A
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..66
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    544..678
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(568..587)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     622..641
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(789..805)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(789..805)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(802..824)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    813..829
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(837..843,844..861,862..867))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    882..903
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctctaaaagc tgcggaattg tacccgcggg cccaccatgt acccatacga tgttccagat
       61 tacgctctta tggccatgga ggcccgaatt cccatgaacc acacttctca agccttcatc
      121 accgctgcca gtggaggaca gcccccaaac tacgaaagaa tcaaggaaga atatgaggtg
      181 gctgagatgg gggcaccgca cggatcggct tctgtcagaa ctactgtgat caacatgccc
      241 agagaggtgt cggtgcctga ccatgtggtc tggtccctgt tcaatacact cttcatgaac
      301 ttctgcgcac tgggcttcat agcctatgcc tactccgtga agtctaggga tcggaagatg
      361 gtgggtgatg tgactggagc ccaggcctac gcctccactg ctaagtgcct gaacatcagc
      421 accttggtcc tcagcatcct gatggttgtt atcaccattg ttagtgtcat catcattgtt
      481 cttaacgctc aaaaccttca cacttaagtc gaccgagatc tctcgaggta ccgcggccgc
      541 ggggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
      601 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
      661 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
      721 gaggtgtggg aggttttttc ggatcctcta gagtcgatct gcaggcatgc tagcttggcg
      781 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      841 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      901 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg cca
//
