LOCUS       sequence-96003-e         866 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses murine IFITM3-deltaY20 with an N-terminal myc tag in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Chesarino NM, McMichael TM, Hach JC, Yount JS
  TITLE     Phosphorylation of the antiviral protein interferon-inducible 
            transmembrane protein 3 (IFITM3) dually regulates its endocytosis 
            and ubiquitination.
  JOURNAL   J Biol Chem. 2014 Apr 25;289(17):11986-92. doi: 
            10.1074/jbc.M114.557694. Epub 2014 Mar 13.
  PUBMED    24627473
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.557694"; journalName: "J Biol Chem"; date: 
            "2014-04-25- 25"; volume: "289"; issue: "17"; pages: "11986-92"
COMMENT     Sequence Label: pCMV-myc-mIFITM3-deltaY20
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    550..684
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(574..593)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     628..647
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(795..811)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(795..811)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(808..830)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    819..835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctctaaaagc tgcggaattg tacccgcggg cccaccatgg catcaatgca gaagctgatc
       61 tcagaggagg acctgcttat ggccatggag gcccgaattc ccatgaacca cacttctcaa
      121 gccttcatca ccgctgccag tggaggacag cccccaaacg aaagaatcaa ggaagaatat
      181 gaggtggctg agatgggggc accgcacgga tcggcttctg tcagaactac tgtgatcaac
      241 atgcccagag aggtgtcggt gcctgaccat gtggtctggt ccctgttcaa tacactcttc
      301 atgaacttct gctgcctggg cttcatagcc tatgcctact ccgtgaagtc tagggatcgg
      361 aagatggtgg gtgatgtgac tggagcccag gcctacgcct ccactgctaa gtgcctgaac
      421 atcagcacct tggtcctcag catcctgatg gttgttatca ccattgttag tgtcatcatc
      481 attgttctta acgctcaaaa ccttcacact taagtcgacc gagatctctc gaggtaccgc
      541 ggccgcgggg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
      601 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
      661 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      721 cagggggagg tgtgggaggt tttttcggat cctctagagt cgatctgcag gcatgctagc
      781 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      841 cacaacatac gagccggaag cataaa
//
