LOCUS       sequence-97088-e        1043 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Probe for Drosophila melanogaster Or22c expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Vosshall LB, Amrein H, Morozov PS, Rzhetsky A, Axel R
  TITLE     A spatial map of olfactory receptor expression in the Drosophila 
            antenna.
  JOURNAL   Cell. 1999 Mar 5;96(5):725-36.
  PUBMED    10089887
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1999-03-5"; volume: "96(5)"; pages: "725-36"
COMMENT     Sequence Label: pGEM-T Dmel Or22c
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..22
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3..21
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      52..61
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(883..901)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(884..901)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(919..935)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(919..935)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(932..954)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    943..959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(967..973,974..991,992..997))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1012..1033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgtaatacga ctcactatag ggcgaattgg gcccgacgtc gcatgctccc ggccgccatg
       61 gccgcgggat ttgggtcttc ttccgctcca atcgccggtg ggcggagttg gtccagcgcc
      121 tgcgggctat tttgtgggaa tcgcggcggc aggaggccca gaggatgctg gtcggactgg
      181 ccaccacggc caacaggctc agcctgttgc tgctcagctc tggcacggcg acaaatgccg
      241 ccttcacctt gcaaccgctg attatgggtc tctaccgctg gattgtgcag ctgccaggtc
      301 aaaccgagct gccctttaat atcatgtgag taagctagtt tgtggtttct aacgctattt
      361 cgtgcaactt tattacttac gtaagcatcg cgtatccgtt aagatgaaaa gttcctcaac
      421 caatttggtt gatggcaggt gatacataag taatgattcc aattgccccc gccagaagca
      481 cttaagatac gccatccgcc attcgccatt aatcgcagtt ccttttaaac agactgccct
      541 cgtttgccgt gcagccagga gtctttccgc tcacctacgt gctgctgacc gcttccggtg
      601 cctgcaccgt tttcgccttc agcttcgtgg acggattctt catttgctcg tgcctctaca
      661 tctgcggcgc tttccggctg gtgcagcagg acattcgcag gatatttgcc gatttgcatg
      721 gcggtgggtc tggctcggct gcggcacgat gacaatggtt ttccctcgct cgatgacttt
      781 ccttggcttt cctttccctt gcagactcaa tcactagtgc ggccgcctgc aggtcgacca
      841 tatgggagag ctcccaacgc gttggatgca tagcttgagt attctatagt gtcacctaaa
      901 tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
      961 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     1021 ctaactcaca ttaattgcgt tgc
//
