LOCUS       sequence-97846-e        1007 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  gRNA expression by the H1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ranganathan V, Wahlin K, Maruotti J, Zack DJ
  TITLE     Expansion of the CRISPR-Cas9 genome targeting space through the use 
            of H1 promoter-expressed guide RNAs.
  JOURNAL   Nat Commun. 2014 Aug 8;5:4516. doi: 10.1038/ncomms5516.
  PUBMED    25105359
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-08-8"; volume: "5:4516. doi"; pages: " 
            10.1038/ncomms5516"
COMMENT     Sequence Label: pH1v1
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..41
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(53..128)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(140..354)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(200..219)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(378..397)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     497..519
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(557..575)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        643..747
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(966..985)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtcgactcta gtaatgccaa ctttgtacaa gaaagctggg tctagaaaaa aagcaccgac
       61 tcggtgccac tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc
      121 tctaaaacct agggaaaaag tggtctcata cagaacttat aagattccca aatccaaaga
      181 catttcacgt ttatggtgat ttcccagaac acatagcgac atgcaaatat tgcagggcgc
      241 cactcccctg tccctcacag ccatcttcct gccagggcgc acgcgcgctg ggtgttcccg
      301 cctagtgaca ctgggcccgc gattccttgg agcgggttga tgacgtcagc gttcgaattc
      361 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
      421 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
      481 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
      541 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
      601 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      661 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      721 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      781 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
      841 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
      901 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
      961 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaaga
//
