LOCUS       sequence-98104-e        1014 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Characteristic fingerprint molecule fused to cohesin type III domain
            to perform single molecule force spectroscopy experiments; 
            covalently immobilized via introduced ybbR tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Schoeler C, Malinowska KH, Bernardi RC, Milles LF, Jobst MA, Durner 
            E, Ott W, Fried DB, Bayer EA, Schulten K, Gaub HE, Nash MA
  TITLE     Ultrastable cellulosome-adhesion complex tightens under load.
  JOURNAL   Nat Commun. 2014 Dec 8;5:5635. doi: 10.1038/ncomms6635.
  PUBMED    25482395
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-12-8"; volume: "5:5635. doi"; pages: " 
            10.1038/ncomms6635"
COMMENT     Sequence Label: pET28a-CohIII-CBM-HIS-ybbR
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             896..913
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             920..943
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 tgatgatttt ggtgctgacg gtgtaatgtg gactgttgaa ctcaaggttc cagcagacgc
       61 taaggctggc gatgtatatc caatcgacgt tgcttatcag tgggatcctt caaagggtga
      121 ccttttcaca gataacaagg actcagcaca gggcaagctg atgcaggctt acttcttcac
      181 acagggtatc aagtcatctt ctaatccttc aacagatgag tacctcgtaa aggctaacgc
      241 tacatatgct gatggttaca ttgcaatcaa ggctggtgag ccaggcagtg tagtaccatc
      301 aacacagcct gtaacaacac cacctgcaac aacaaaacca cctgcaacaa caataccgcc
      361 gtcagatgat ccgaatgcca tggccaatac accggtatca ggcaatttga aggttgaatt
      421 ctacaacagc aatccttcag atactactaa ctcaatcaat cctcagttca aggttactaa
      481 taccggaagc agtgcaattg atttgtccaa actcacattg agatattatt atacagtaga
      541 cggtcagaaa gatcagacct tctggagtga ccatgctgca ataatcggca gtaacggcag
      601 ctacaacgga attacttcaa atgtaaaagg aacatttgta aaaatgagtt cctcaacaaa
      661 taacgcagac acctaccttg aaataagctt tacaggcgga actcttgaac cgggtgcaca
      721 tgttcagata caaggtagat ttgcaaagaa tgactggagt aactatacac agtcaaatga
      781 ctactcattc aagtctgctt cacagtttgt tgaatgggat caggtaacag catacttgaa
      841 cggtgttctt gtatggggta aagaacccgg tgagctcaag cttcctaggt ctaggcacca
      901 ccaccaccac cacggatccc tggaagttct gttccagggt ccggactctc tggaattcat
      961 cgcttctaaa ctggcttaac tcgagtaaga tccggctgct aacaaagccc gaaa
//
