LOCUS       sequence-98111-e         960 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Luciferase reporter construct containing three hypoxia response 
            elements (24-mers) from the Pgk-1 gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Emerling BM, Weinberg F, Liu JL, Mak TW, Chandel NS
  TITLE     PTEN regulates p300-dependent hypoxia-inducible factor 1 
            transcriptional activity through Forkhead transcription factor 3a 
            (FOXO3a).
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Feb 19. 105(7):2622-7.
  PUBMED    18268343
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Feb 19. 105(7):2622-7."
COMMENT     Sequence Label: HRE-luciferase
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    314..435
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(351..370)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     405..424
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        636..698
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     primer_bind     complement(824..842)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
       61 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
      121 atttgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
      181 tcgctattac gccagcccaa gctaccatga taagtaagta atattaaggt acgtggaggt
      241 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
      301 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
      361 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
      421 catcaatgta tcttatggta ctgtaactga gctaacataa cccgggaggt accgagctct
      481 gtcacgtcct gcacgactct agttgtcacg tcctgcacga ctctagttgt cacgtcctgc
      541 acgacgctag ctcgagatcc ggccccgccc agcgtcttgt cattggcgaa ttcgaacacg
      601 cagatgcagt cggggcggcg cggtccgagg tccacttcgc atattaaggt gacgcgtgtg
      661 gcctcgaaca ccgagcgacc ctgcagcgac ccgcttaaca gcgtcaacag cgtgccgcag
      721 atctaagtaa gcttggcatt ccggtactgt tggtaaaatg gaagacgcca aaaacataaa
      781 gaaaggcccg gcgccattct atcctctaga ggatggaacc gctggagagc aactgcataa
      841 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga
      901 ggtgaacatc acgtacgcgg aatacttcga aatgtccgtt cggttggcag aagctatgaa
//
