LOCUS       sequence-98126-e         735 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Labels synaptic vesicles for electron microscopy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Atasoy D, Betley JN, Li WP, Su HH, Sertel SM, Scheffer LK, Simpson 
            JH, Fetter RD, Sternson SM
  TITLE     A genetically specified connectomics approach applied to long-range 
            feeding regulatory circuits.
  JOURNAL   Nat Neurosci. 2014 Dec;17(12):1830-9. doi: 10.1038/nn.3854. Epub 
            2014 Nov 2.
  PUBMED    25362474
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3854"; 
            journalName: "Nat Neurosci"; date: "2014-12"; volume: "17"; issue: 
            "12"; pages: "1830-9"
COMMENT     Sequence Label: AAV-FLEX-VAMP2:HRP
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..81
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     143..162
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    197..230
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    293..326
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 acggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg
       61 ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc
      121 ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc
      181 aaagaattgg atccccataa cttcgtataa agtatcctat acgaagttat atcaaaatag
      241 gaagaccaat gcttcaccat cgacccgaat tgccaagcat caccatcgac ccataacttc
      301 gtataatgta tgctatacga agttatacta ggctaagagt tgctgttgac cactctgcag
      361 ttcagacgaa tctggccttg cgtacccgtc agaggggtaa tgttacccat acggtccatg
      421 gcttccacga aggcgttaaa gaaggtttga gtagagttag caaaacttct caccagtggg
      481 atggtgtcag tggcgtttgg actgccaaac agttcttgat cactctgtat caggcctttc
      541 tgctcctcta gattcacata gtacttgtta tcgaagatgg ttggggtccg cagatcaaag
      601 tccactagtg cactgaggtt gccattcagt gggcacaagc ctctcagtgt ctggagatac
      661 gtagtgttca acgtggggtc aggtaaccca gtgttgctga aattgtagag cctatccatg
      721 atgaacctac actgg
//
