LOCUS       sequence-98130-e       11300 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses CTIP2 under the EF1a promoter .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11300)
  AUTHORS   Victor MB, Richner M, Hermanstyne TO, Ransdell JL, Sobieski C, Deng 
            PY, Klyachko VA, Nerbonne JM, Yoo AS
  TITLE     Generation of Human Striatal Neurons by MicroRNA-Dependent Direct 
            Conversion of Fibroblasts.
  JOURNAL   Neuron. 2014 Oct 22;84(2):311-23. doi: 10.1016/j.neuron.2014.10.016.
            Epub 2014 Oct 22.
  PUBMED    25374357
REFERENCE   2  (bases 1 to 11300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.10.016"; journalName: "Neuron"; date: 
            "2014-10-22- 22"; volume: "84"; issue: "2"; pages: "311-23"
COMMENT     Sequence Label: pmCTIP2-N174
FEATURES             Location/Qualifiers
     source          1..11300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        5878..6377
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5913..5936)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6310..6329
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6404..7198
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6404..7198
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6458..6477)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7068..7087
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7222..7810
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7275..7295)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7693..7704)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8018..8651
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8780..8796)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8780..8796)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8793..8815)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8804..8820
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8828..8834,8835..8852,8853..8858))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8873..8894
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9011..9028)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9182..9767)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9262..9281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9938..10729,10730..10798))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10561..10580
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10799..10903)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10951..11085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10988..11007)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11042..11061
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcgg ccgccaccat gtcccgccgc aaacagggca
     3421 acccgcagca cttgtcccag agggaactca tcacgccaga ggctgaccat gtggaggcta
     3481 ccatcctcga ggaagacgag ggtctggaga tagaggagcc tagcagcctg gggctgatgg
     3541 tgggaggccc cgaccctgat ctactcacct gtggccagtg tcagatgaac ttcccgctgg
     3601 gggacatcct ggtttttata gagcacaaga agaaacagtg tggaggcctg ggcccctgct
     3661 acgacaaggt cctggacaag agcagtccac ctccctcctc tcgctctgag ctcaggagag
     3721 tatctgagcc agtggagatc gggatccagg tcacccctga tgaagatgac cacctactgt
     3781 cacccacgaa aggcatctgt cccaagcagg agaacattgc aggtaaagat gagccttcca
     3841 gctacatttg cacaacatgc aagcagccct tcaacagcgc ctggttcctg ctgcagcacg
     3901 cacagaacac gcatggcttc cgaatctacc tggagcctgg gccggccagc acctcgctca
     3961 cgcccaggct caccatcccg ccaccgctcg ggccggagac cgtggcgcag tccccactca
     4021 tgaatttcct gggggacagc aatcctttca acctgctgcg catgacgggc cccatcctgc
     4081 gggaccaccc tggcttcggt gagggccgct tgccaggtac gccaccgctc ttcagcccac
     4141 cgccacgcca tcacttggac ccacaccgcc tcagtgcaga ggagatgggg ctcgtggccc
     4201 agcaccccag tgccttcgac cgagtcatgc gcctgaaccc catggccata gactctcctg
     4261 ccatggactt ctcccggcgg ctgcgagaac tggccggcaa cagctccacg ccgccgcccg
     4321 tgtccccagg ccgtggcaac cctatgcacc ggctgctgaa ccctttccag cccagtccca
     4381 agtccccgtt cctcagcacg ccaccgctgc cacccatgcc tgcgggcaca ccgccaccgc
     4441 agccgcctgc caagagcaag tcctgtgagt tctgcggcaa gaccttcaag ttccagagca
     4501 atctcatcgt gcaccggcgc agccacacgg gcgagaagcc ctacaagtgc cagctgtgcg
     4561 accatgcgtg ctcgcaggcg agcaagctca agcgccacat gaagacgcac atgcacaagg
     4621 cgggctctct ggctggccgc tcagacgacg ggctctcagc tgccagctcc cctgagccgg
     4681 gcaccagcga gctgccaggt gacctgaaag cggccgatgg cgacttccgc caccatgaga
     4741 gcgacccatc tctgggcccc gagcctgagg acgacgagga cgaggaggag gaagaagagg
     4801 agctgctgct ggagaacgag agccggcctg agtcgagctt cagcatggac tcggagctgg
     4861 gccgtggccg cgagaacgga ggtggtgtgc caccgggggt ggcgggcgca ggggctgcag
     4921 ctgcggctct ggcggatgag aaggctctgg ccctgggcaa ggtgatggag gacgcagggc
     4981 tgggcgcact gccgcagtat ggggagaagc ggggcgcctt cctgaagcgt gcaggcgaca
     5041 cgggtgatgc cggagctgtt ggctgtgggg acgcgggtgc accgggtgca gtgaacgggc
     5101 gcggcggggc cttcgcgcca ggcgcagagc cctttccagc tctcttccca cgcaagccag
     5161 caccgctgcc cagccctggg ctcggtggtc ccgcgctgca cgcggccaag cgcatcaagg
     5221 tggagaaaga cctggagctg ccacctgccg ccctcatccc atctgagaac gtgtactcgc
     5281 agtggctcgt gggctacgca gcatcgcgcc acttcatgaa ggacccattc ctgggcttca
     5341 cggatgcgcg ccagtcgcct ttcgccacat cgtcggaaca ttcctctgag aacggcagcc
     5401 tgcgcttctc aacgccaccc ggggacctgc tggacggcgg gctgtccggg cgcagtggca
     5461 cggcgagcgg gggcagcaca cctcacctgg gtggtccggg tcctgggagg ccgagctcca
     5521 aggagggccg ccgcagcgac acatgtgagt actgcggcaa ggtcttcaag aactgtagca
     5581 acctgacggt gcaccggagg agccacaccg gcgagcggcc ttacaagtgc gagctgtgca
     5641 actacgcgtg tgcgcagagc agcaagctca cgcgccacat gaagacgcac gggcagatcg
     5701 gcaaggaggt gtaccgctgc gacatctgcc agatgccctt cagcgtctac agcaccctgg
     5761 agaaacacat gaaaaagtgg cacggtgaac acttgctgac taatgatgtc aaaatcgagc
     5821 aggctgagag gagctaaggc cgcgccggct ctagatcgcg aacgcgtgaa ttctaccggg
     5881 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     5941 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     6001 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     6061 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     6121 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     6181 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     6241 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     6301 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     6361 catctccggg cctttcgacc tgcagcccaa gcttcctagg accatgattg aacaagatgg
     6421 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     6481 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     6541 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     6601 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     6661 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     6721 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     6781 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     6841 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     6901 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     6961 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     7021 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     7081 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     7141 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgacc
     7201 cgggcggggc gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact
     7261 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7321 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7381 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7441 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7501 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7561 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     7621 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7681 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7741 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7801 gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa
     7861 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     7921 aggtgggttt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     7981 gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa
     8041 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
     8101 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
     8161 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
     8221 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
     8281 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
     8341 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
     8401 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
     8461 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8521 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     8581 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8641 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     8701 atgaatatca gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg acctctagct
     8761 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8821 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8881 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8941 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     9001 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     9061 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     9121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     9661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt
     9721 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9781 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9841 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9901 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9961 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    10021 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    10081 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    10141 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    10201 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    10261 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    10321 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    10381 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10441 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10501 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10561 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10621 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10681 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10741 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10801 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10861 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10921 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac
    10981 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    11041 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    11101 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattaccta
    11161 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
    11221 tatttgttaa atatgtacta caaacttagt agtttttaaa gaaattgtat ttgttaaata
    11281 tgtactacaa acttagtagt
//
