LOCUS       sequence-98190-e         245 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  PT7 GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-07-30"; volume: "10(7)"; pages: "742"
COMMENT     Sequence Label: pTHSSd_8
FEATURES             Location/Qualifiers
     source          1..245
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     145..163
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     184..203
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        184..202
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
       61 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccgacgtcta agaaaccatt
      121 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcacctc
      181 gagtaatacg actcactata gggagaggat cctgtcaatt tccgcgatag aggaggtaaa
      241 gatga
//
