LOCUS       sequence-98214-e         752 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Knocks down TACC3 in mammalian cells via shRNA and co-expresses 
            GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Booth DG, Hood FE, Prior IA, Royle SJ
  TITLE     A TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by 
            inter-microtubule bridging.
  JOURNAL   EMBO J. 2011 Mar 2;30(5):906-19. doi: 10.1038/emboj.2011.15. Epub 
            2011 Feb 4.
  PUBMED    21297582
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2011.15"; journalName: "EMBO J"; date: "2011-03-2- 
            2"; volume: "30"; issue: "5"; pages: "906-19"
COMMENT     Sequence Label: pBrain-GFP-shTACC3
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..62
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     46..62
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        82..296
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     217..236
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(369..385)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(370..386)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(414..432)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(453..469)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(453..469)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(466..488)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    477..493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(501..507,508..525,526..531))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    546..567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gactcactat agggcgaatt ggagctccac cgcggtggcg gccgctctag aactagtgga
       61 tcccccgggc tgcaggaatt cgaacgctga cgtcatcaac ccgctccaag gaatcgcggg
      121 cccagtgtca ctaggcggga acacccagcg cgcgtgcgcc ctggcaggaa gatggctgtg
      181 agggacaggg gagtggcgcc ctgcaatatt tgcatgtcgc tatgtgttct gggaaatcac
      241 cataaacgtg aaatgtcttt ggatttggga atcttataag ttctgtatga gaccacagat
      301 ccccgttacc ggaagatcgt ctgttcaaga gacagacgat cttccggtaa ctttttggaa
      361 aagcttatcg ataccgtcga cctcgagggg gggcccggta ccagcttttg ttccctttag
      421 tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      481 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      541 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      601 ggaaacctgt cgtgccagat agtaatcaat tacggggtca ttagttcata gcccatatat
      661 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      721 ccgcccattg acgtcaataa tgacgtatgt tc
//
