LOCUS       sequence-98215-e         862 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Disintegrator plasmid encoding MET17 promoter driven red beetle 
            luciferase with nuclear localization signal (NLS) and ClpXP degron 
            tag (ssrA)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     ""Measuring fast gene dynamics in single cells with timelapse 
            luminescence microscopy""
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB697
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..64
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 attctattac ccccatccat actcttaatt aaccatggct ccaccaaaaa aaaaaagaaa
       61 agttgccatg ggtgggggca gcatggaaga agaaaacatt gtgaatggag atcgtcctcg
      121 tgatctagtt tttcctggca cagcaggact acaattatat caatcattat ataaatattc
      181 atatattact gacggaataa tcgatgccca taccaatgaa gtaatatcat atgctcaaat
      241 atttgaaacc agctgccgct tggcagttag tctagaaaaa tatggcttgg atcataacaa
      301 tgttgtggca atatgcagtg aaaacaacat acactttttt ggccctttaa ttgctgcttt
      361 ataccaagga ataccaatgg caacatcaaa tgatatgtat acagaaaggg agatgattgg
      421 ccatttgaat atatcgaaac catgccttat gttttgttca aagaaatcac tcccatttat
      481 tctgaaagta caaaaacatc tagatttcct taaaaaagtc atagtcattg atagtatgta
      541 cgacatcaat ggcgttgaat gcgtatttag ctttgtttca cgttatactg atcacgcctt
      601 tgatccagtg aaatttaacc caaaagagtt tgatcccttg gaaagaaccg cattaattat
      661 gacatcatct ggaacaactg gattgcctaa aggggtagta ataagccata gaagtataac
      721 tataagattc gtccatagca gtgatcccat ctatggtact cgtattgctc cagatacatc
      781 aattcttgct atagcaccgt tccatcatgc ctttggactg tttactgcac tagcttactt
      841 tccagtagga cttaagattg ta
//
