LOCUS       sequence-98219-e         618 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A 
            substitution and knocks down endogenous TACC3 via shRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Booth DG, Hood FE, Prior IA, Royle SJ
  TITLE     A TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by 
            inter-microtubule bridging.
  JOURNAL   EMBO J. 2011 Mar 2;30(5):906-19. doi: 10.1038/emboj.2011.15. Epub 
            2011 Feb 4.
  PUBMED    21297582
REFERENCE   2  (bases 1 to 618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2011.15"; journalName: "EMBO J"; date: "2011-03-2- 
            2"; volume: "30"; issue: "5"; pages: "906-19"
COMMENT     Sequence Label: pBrain-GFP-TACC3KDP(S558A)-shTACC3
FEATURES             Location/Qualifiers
     source          1..618
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..63
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     47..63
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        83..297
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     218..237
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(370..386)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(371..387)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(415..433)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(454..470)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(454..470)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(467..489)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    478..494
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(502..508,509..526,527..532))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    547..568
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg
       61 atcccccggg ctgcaggaat tcgaacgctg acgtcatcaa cccgctccaa ggaatcgcgg
      121 gcccagtgtc actaggcggg aacacccagc gcgcgtgcgc cctggcagga agatggctgt
      181 gagggacagg ggagtggcgc cctgcaatat ttgcatgtcg ctatgtgttc tgggaaatca
      241 ccataaacgt gaaatgtctt tggatttggg aatcttataa gttctgtatg agaccacaga
      301 tccccgttac cggaagatcg tctgttcaag agacagacga tcttccggta actttttgga
      361 aaagcttatc gataccgtcg acctcgaggg ggggcccggt accagctttt gttcccttta
      421 gtgagggtta atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
      481 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
      541 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
      601 gggaaacctg tcgtgcca
//
