LOCUS       sequence-98477-e         690 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Recombinant protein expression of YbbN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Lin J, Wilson MA
  TITLE     Escherichia coli thioredoxin-like protein YbbN contains an atypical 
            tetratricopeptide repeat motif and is a negative regulator of GroEL.
  JOURNAL   J Biol Chem. 2011 Jun 3;286(22):19459-69. doi: 
            10.1074/jbc.M111.238741. Epub 2011 Apr 15.
  PUBMED    21498507
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.238741"; journalName: "J Biol Chem"; date: 
            "2011-06-3- 3"; volume: "286"; issue: "22"; pages: "19459-69"
COMMENT     Sequence Label: EcYbbN pET15b
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..86
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca
       61 gcagcggcct ggtgccgcgc ggcagccata tgtccgtaga aaatattgtc aacattaacg
      121 aatctaacct gcaacaggtt cttgaacagt cgatgaccac tccggtgctg ttctattttt
      181 ggtctgaacg tagccagcac tgtttgcagt taaccccgat cctggaaagc ctcgcggcgc
      241 agtacaacgg gcaatttatt ctggcgaagc tggactgcga cgcggagcag atgattgccg
      301 cccagtttgg tctgcgcgcg attccgaccg tgtatctgtt ccagaacggg caaccggtag
      361 atggcttcca ggggccgcaa ccggaagagg cgatccgcgc cctgctggat aaagtgctgc
      421 cgcgcgaaga agagctgaaa gcgcagcagg cgatgcaact gatgcaggaa agcaattaca
      481 ccgatgccct gccattgctg aaagacgcct ggcagttgtc gaatcagaac ggggagatcg
      541 gcctgctgct ggcagaaacg ctgattgcgc tgaaccgttc tgaagatgcg gaagcggtgc
      601 tgaaaaccat tccgttgcag gatcaggaca cccgctacca ggggctggtg gcgcaaatcg
      661 aactgctgaa gcaggcggct gatactccgg
//
