LOCUS       sequence-98557-e         822 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expression of yeast beta tubulin (TUB2)-Cterminal 6xHis using a GAL 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS424-TUB2-Cterminal6xHis
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(20..267)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     233..251
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     269..285
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     270..286
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(316..333)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             728..736
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gggcgaatgg gtaccggccg caaattaaag ccttcgagcg tcccaaaacc ttctcaagca
       61 aggttttcag tataatgtta catgcgtaca cgcgtctgta cagaaaaaaa agaaaaattt
      121 gaaatataaa taacgttctt aatactaaca taactataaa aaaataaata gggacctaga
      181 cttcaggttg tctaactcct tccttttcgg ttagagcgga tgtgggggga gggcgtgaat
      241 gtaagcgtga cataactaat tacatgactc gaggtcgacg gtatcgataa gcttgatatc
      301 gaattcctgc agttagtggt ggtggtggtg gtgttcaaaa ttctcagtga ttggttcatc
      361 ttggttttgt ggagcaccaa aatcgccatt ttcgtcgact tcttcatcat cttctacagt
      421 agcctcttgg tattgttggt attcgctaac cagatcattc atattagatt cagcctcaga
      481 gaattccaat tcgtccatac cttcactagt ataccagtgc aagaaagctt ttcttttgaa
      541 catagcggaa aattggtcac caactctctt gaatagctct tgaatagatg tggagttagc
      601 aatgaaagta gcagccatgt ccaaaccttg aggagcgaca gaacacacag cagtttgcac
      661 attgttgggg atccattcca cgaaatagtc tgagttttta gattgcactt tatgcatttc
      721 atcttccacc tccttaacgg aaactttacc tctaaagaag gctgcaacgg taaggtatct
      781 accgtttctt ggatcggcag cagccatcat gttcttggca tc
//
