LOCUS       sequence-98890-e         577 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Q30D mutant of the mouse MSI1 RBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, 
            Ryder SP
  TITLE     Allosteric inhibition of a stem cell RNA-binding protein by an 
            intermediary metabolite.
  JOURNAL   Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848.
  PUBMED    24935936
REFERENCE   2  (bases 1 to 577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            Jun 16:e02848. doi: 10.7554/eLife.02848."
COMMENT     Sequence Label: pET-22HT-MSI1(7-192)Q30D
FEATURES             Location/Qualifiers
     source          1..577
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..34
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             38..58
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aggagatata catatgcatc atcatcatca tcacggcgaa aacctgtatt ttcagggcgg
       61 atcccagccc ggcctcgcct ccccggactc gccgcacgac ccctgcaaga tgttcatcgg
      121 aggactcagt tgggatacca cgcaggaagg gctgcgcgaa tacttcggcc agttcgggga
      181 ggtgaaagag tgtctggtga tgcgggaccc cctgaccaaa agatccaggg gtttcggctt
      241 cgtcactttc atggaccagg cgggggtgga taaagtgctg gcgcaatcgc ggcacgagct
      301 cgactccaaa acaattgacc ccaaggtggc ctttcctcga agagcacagc ctaagatggt
      361 cactcggacg aagaagatct tcgtgggggg gctgtctgtg aacaccacgg tggaagatgt
      421 gaaacactat ttcgagcagt tcggaaaggt ggatgatgcc atgctgatgt tcgacaaaac
      481 caccaacagg cacagagggt ttggatttgt cacgtttgag agcgaggaca tcgtagagaa
      541 agtttgtgag atccacttcc atgaaatcaa caacaaa
//
