LOCUS       sequence-98891-e         650 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  G35D mutant of the mouse MSI1 RBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, 
            Ryder SP
  TITLE     Allosteric inhibition of a stem cell RNA-binding protein by an 
            intermediary metabolite.
  JOURNAL   Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848.
  PUBMED    24935936
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            Jun 16:e02848. doi: 10.7554/eLife.02848."
COMMENT     Sequence Label: pET-22HT-MSI1(7-192)G35D
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..47
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             51..71
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tttaacttta agaaggagat atacatatgc atcatcatca tcatcacggc gaaaacctgt
       61 attttcaggg cggatcccag cccggcctcg cctccccgga ctcgccgcac gacccctgca
      121 agatgttcat cggaggactc agttggcaga ccacgcagga agatctgcgc gaatacttcg
      181 gccagttcgg ggaggtgaaa gagtgtctgg tgatgcggga ccccctgacc aaaagatcca
      241 ggggtttcgg cttcgtcact ttcatggacc aggcgggggt ggataaagtg ctggcgcaat
      301 cgcggcacga gctcgactcc aaaacaattg accccaaggt ggcctttcct cgaagagcac
      361 agcctaagat ggtcactcgg acgaagaaga tcttcgtggg ggggctgtct gtgaacacca
      421 cggtggaaga tgtgaaacac tatttcgagc agttcggaaa ggtggatgat gccatgctga
      481 tgttcgacaa aaccaccaac aggcacagag ggtttggatt tgtcacgttt gagagcgagg
      541 acatcgtaga gaaagtttgt gagatccact tccatgaaat caacaacaaa atggtggaat
      601 gcaagaaagc ccagccaaag gaggtgatgt ccccgtgaaa gcttgcggcc
//
