LOCUS       sequence-99298-e         773 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  ""CoinFLP-LexGAD/Gal4"" - Expresses LexGAD or Gal4 from actin 
            promoter downsteam of transcriptional stop. Recombination by FLP 
            excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express
            Gal4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Bosch JA, Tran NH, Hariharan IK
  TITLE     CoinFLP: a system for efficient mosaic screening and for visualizing
            clonal boundaries in Drosophila.
  JOURNAL   Development. 2015 Feb 1;142(3):597-606. doi: 10.1242/dev.114603.
  PUBMED    25605786
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2015-02-1- 1"; volume: "142"; 
            issue: "3"; pages: "597-606"
COMMENT     Sequence Label: pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          406..471
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             601..621
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgatagctca ttgtccttca ctttcactaa cagtagcaac ggtccgaacc tcataacaac
       61 tcaaacaaat tctcaagcgc tttcacaacc aattgcctcc tctaacgttc atgataactt
      121 catgaataat gaaatcacgg ctagtaaaat tgatgatggt aataattcaa aaccactgtc
      181 acctggttgg acggaccaaa ctgcgtataa cgcgtttgga atcactacag ggatgtttaa
      241 taccactaca atggatgatg tatataacta tctattcgat gatgaagata ccccaccaaa
      301 cccaaaaaaa gagtaaggta cctctagagg atctttgtga aggaacctta cttctgtggt
      361 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
      421 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
      481 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
      541 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
      601 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
      661 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
      721 gaaaaagctg cactgctata caagaaaatt atggaaaaat atttgatgta tag
//
