LOCUS       sequence-99875-e         885 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  expresses an insert when Cre is expressed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  TITLE     Birnbaum lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLPNPX1
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..105
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(135..551)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(151..167)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(183..205)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatccg
       61 ctagcagtta aataacttcg tataatgtat gctatacgaa gttataccgg ttaaatggat
      121 cagatcggaa ttccggcgcc tagagaagga gtgagggctg gataaaggga ggatcgaggc
      181 ggggtcgaac gaggaggttc aagggggaga gacggggcgg atggaggaag aggaggcgga
      241 ggcttagggt gtacaaaggg cttgacccag ggaggggggt caaaagccaa ggcttcccag
      301 gtcacgatgt aggggacctg gtctgggtgt ccatgcgggc caggtgaaaa gaccttgatc
      361 ttaacctggg tgatgaggtc tcggttaaag gtgccgtctc gcggccatcc gacgttaaag
      421 gttggccatt ctgcagagca gaaggtaacc caacgtctct tcttgacatc taccgactgg
      481 ttgtgagcga tccgctcgac atctttccag tgacctaagg tcaaacttaa gggagtggta
      541 acagtctggc cctaattttc agacaaatac agaaacacag tcagacagag acaacacaga
      601 acgatgctgc agcgctgcag cagacaagac gcgcggcttc ggttccaaac cgaaagcaaa
      661 aattcagacg gaggcgggaa ctgttttagg ttctcgtctc ctaccagaac cacatatcct
      721 gacggggtcg gattccacat cgactccctt cctcaggtcg ggccacaaaa acggccccca
      781 aagtccctgg gacgtctccc agggttgcgg ccgggtgttc agaactcgtc agttccacca
      841 cgggtccgcc agatacagag ctagttagct aactagtaca gacgc
//
