LOCUS       sequence-99885-e         651 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  A lentiviral plasmid that encodes both a sgRNA targeting CDKN1B and 
            BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pLV-sgCDKN1B#2 BFP
FEATURES             Location/Qualifiers
     source          1..651
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(296..329)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     333..353
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
       61 cagcagagat ccagtttggt tagtaccggg cccgctctag agatccgacg cgccatctct
      121 aggcccgcgc cggccccctc gcacggactt gtgggagaag ctcggctact cccctgcccc
      181 ggttaatttg catataatat ttcctagtaa ctatagaggc ttaatgtgcg ataaaagaca
      241 gataatctgt tctttttaat actagctaca ttttacatga taggcttgga tttctataac
      301 ttcgtatagc atacattata cgaagttata aacagcacaa aaggaaactc accctaactg
      361 taaagtaatt gtgtgttttg agactataag tatcccttgg agaaccacct tgttgggaag
      421 ccgggacctg gaccaggttt aagagctaag ctggaaacag catagcaagt ttaaataagg
      481 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttc tcgagtacta
      541 ggatccatta ggcggccgcg tggataaccg tattaccgcc atgcatgtgc ccgtcagtgg
      601 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg c
//
