LOCUS       sequence-100050-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-PCNA-19-NLS-4
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             59..79
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      92..101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..805
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(243..261)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     511..530
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagccacc atggcttcgt ggggctcccc
       61 gaagaagaag cgcaaagtac tggtaccggt cgccaccatg gtgagcaagg gcgaggagga
      121 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
      181 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
      241 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
      301 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
      361 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
      421 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
      481 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
      541 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
      601 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
      661 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
      721 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
      781 cggcggcatg gacgagctgt acaagtccgg agaagggcaa gggcaagggc aagggccggg
      841 ccgcggctac gcgtatcgat ccatgttcga ggcgcgcctg gtccagg
//
