LOCUS       sequence-100128-         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-RMCE, second step attB plasmid, intron phase 0, exchange 
            dsRed with flip-out conditional cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Zhang X, Koolhaas WH, Schnorrer F
  TITLE     A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient 
            Genome Engineering in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979.
  PUBMED    25324299
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979."
COMMENT     Sequence Label: pBS-KS-attB1-2-PT-SA-SD-0-FRT-2xTY1-FRT-V5
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    35..104
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    271..318
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    384..431
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             433..474
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    662..731
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 cgaattggag ctcgctagcg atgtaggtca cggtctcgaa gccgcggtgc gggtgccagg
       61 gcgtgccctt gggctccccg ggcgcgtact ccacctcacc catctggtcc atcatgatgg
      121 tcgactctag aagtcgatcc aacatggcga cttgtcccat ccccggcatg tttaaatata
      181 ctaattattc ttgaactaat tttaatcaac cgatttatct ctcttccgca ggtgggaggt
      241 tccggtggaa gcggaggtag cggcggatcg gaagttccta ttccgaagtt cctattctct
      301 agaaagtata ggaacttcga ggtccacact aatcaagacc ccctggatgc cgaggtgcac
      361 accaaccagg accctctgga ccggaagttc ctattccgaa gttcctattc tctagaaagt
      421 ataggaactt cgggcaagcc catccccaac cccctgctgg gcctggatag caccagatcc
      481 ggaggtagcg gtggaagcgg aggttccggc gaggtaagtt attgaacaat ggcatcaaat
      541 gccttcatca tcactaccct ttagccctta agaccccaca atgaccttac ccactcagag
      601 aaaaaagtaa atatgaaagc ccatttgaac ttctaagctt gtcgaccatc atgatggacc
      661 agatgggtga ggtggagtac gcgcccgggg agcccaaggg cacgccctgg cacccgcacc
      721 gcggcttcga gaccgtgacc tacatc
//
