LOCUS       sequence-100255-         664 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses D10A mutant (nickase) of human codon-optimized Cas9 
            (derived from Streptococcus pyogenes) and pruomycin resistance 
            gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Li HL, Fujimoto N, Sasakawa N, Shirai S, Ohkame T, Sakuma T, Tanaka 
            M, Amano N, Watanabe A, Sakurai H, Yamamoto T, Yamanaka S, Hotta A
  TITLE     Precise Correction of the Dystrophin Gene in Duchenne Muscular 
            Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and 
            CRISPR-Cas9.
  JOURNAL   Stem Cell Reports. 2014 Nov 25. pii: S2213-6711(14)00335-X. doi: 
            10.1016/j.stemcr.2014.10.013.
  PUBMED    25434822
REFERENCE   2  (bases 1 to 664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2014 Nov 25. pii: S2213-6711(14)00335-X. doi: 
            10.1016/j.stemcr.2014.10.013."
COMMENT     Sequence Label: pHL-EF1a-SphcCas9(D10A)-iP-A
FEATURES             Location/Qualifiers
     source          1..664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..44
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attagcggtg gcggccatca caagtttgta caaaaaagca ggctggcgcc ggaaccaatt
       61 cagtcgacca tggataagaa atacagcatt ggactggcca ttgggacaaa ctccgtggga
      121 tgggccgtga ttacagacga atacaaagtg ccttcaaaga agttcaaggt gctgggcaac
      181 accgatagac acagcatcaa gaaaaatctg attggagccc tgctgttcga ctccggcgag
      241 acagctgaag caactcggct gaaaagaact gctcggagaa ggtatacccg ccgaaagaat
      301 aggatctgct acctgcagga gattttcagc aacgaaatgg ccaaggtgga cgatagtttc
      361 tttcaccgcc tggaggaatc attcctggtc gaggaagata agaaacacga gcggcatccc
      421 atctttggca acattgtgga cgaggtcgct tatcacgaaa agtaccctac catctatcat
      481 ctgaggaaga aactggtgga ctccacagat aaagcagacc tgcgcctgat ctatctggcc
      541 ctggctcaca tgattaagtt ccggggccat tttctgatcg agggggatct gaacccagac
      601 aattctgatg tggacaagct gttcatccag ctggtccaga catacaatca gctgtttgag
      661 gaaa
//
