LOCUS       sequence-100274-        1094 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression vector for Cre-recombinase containing URA3 
            selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   MacDonald C, Piper RC
  TITLE     Puromycin and Methotrexate Resistance Cassettes and Optimized 
            cre-recombinase Expression Plasmids for use in Yeast.
  JOURNAL   Yeast. 2015 Feb 16. doi: 10.1002/yea.3069.
  PUBMED    25688547
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Feb 16. doi: 10.1002/yea.3069."
COMMENT     Sequence Label: pPL5071_TEF1*-Cre_URA3
FEATURES             Location/Qualifiers
     source          1..1094
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..216
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             217..1020
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 ttcaattcat catttttttt ttattctttt ttttgatttc ggtttccttg aaattttttt
       61 gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg agcacagact tagattggta
      121 tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg
      181 cacagaacaa aaacctgcag gaaacgaaga taaatcatgt cgaaagctac atataaggaa
      241 cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag
      301 caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt
      361 gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt
      421 tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc
      481 ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt
      541 gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt
      601 attgttagcg gtttgaagca ggcggcagaa gaagtaacaa aggaacctag aggccttttg
      661 atgttagcag aattgtcatg caagggctcc ctatctactg gagaatatac taagggtact
      721 gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg
      781 ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac
      841 aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct
      901 gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt
      961 gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa
     1021 aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa
     1081 ttatatcagt tatt
//
