LOCUS       sequence-100275-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to overexpress lamprey deaminase in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Lada AG, Stepchenkova EI, Waisertreiger IS, Noskov VN, Dhar A, Eudy 
            JD, Boissy RJ, Hirano M, Rogozin IB, Pavlov YI
  TITLE     Genome-wide mutation avalanches induced in diploid yeast cells by a 
            base analog or an APOBEC deaminase.
  JOURNAL   PLoS Genet. 2013;9(9):e1003736. doi: 10.1371/journal.pgen.1003736. 
            Epub 2013 Sep 5.
  PUBMED    24039593
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2013"; volume: "9(9):e1003736. doi"; pages: " 
            10.1371/journal.pgen.1003736. Epub 2013 Sep 5"
COMMENT     Sequence Label: pESC-LEU-PmCDA1
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             64..687
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     CDS             700..723
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(926..950)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 catccaaaaa aaaagtaaga atttttgaaa attcgaattc aaccctcact aaagggcggc
       61 cgcatgaccg acgctgagta cgtgagaatc catgagaagt tggacatcta cacgtttaag
      121 aaacagtttt tcaacaacaa aaaatccgtg tcgcatagat gctacgttct ctttgaatta
      181 aaacgacggg gtgaacgtag agcgtgtttt tggggctatg ctgtgaataa accacagagc
      241 gggacagaac gtggaattca cgccgaaatc tttagcatta gaaaagtcga agaatacctg
      301 cgcgacaacc ccggacaatt cacgataaat tggtactcat cctggagtcc ttgtgcagat
      361 tgcgctgaaa agatcttaga atggtataac caggagctgc gggggaacgg ccacactttg
      421 aaaatctggg cttgcaaact ctattacgag aaaaatgcga ggaatcaaat tgggctgtgg
      481 aacctcagag ataacggggt tgggttgaat gtaatggtaa gtgaacacta ccaatgttgc
      541 aggaaaatat tcatccaatc gtcgcacaat caattgaatg agaatagatg gcttgagaag
      601 actttgaagc gagctgaaaa acgacggagc gagttgtcca ttatgattca ggtaaaaata
      661 ctccacacca ctaagagtcc tgctgtacta gtatcgatgg attacaagga tgacgacgat
      721 aagatctgag ctcttaatta acaattcttc gccagaggtt tggtcaagtc tccaatcaag
      781 gttgtcggct tgtctacctt gccagaaatt tacgaaaaga tggaaaaggg tcaaatcgtt
      841 ggtagatacg ttgttgacac ttctaaataa gcgaatttct tatgatttat gatttttatt
      901 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
      961 aaacgaaaat tcttattctt ga
//
