LOCUS       sequence-100281-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression vector for Cre-recombinase containing URA3 
            selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   MacDonald C, Piper RC
  TITLE     Puromycin and Methotrexate Resistance Cassettes and Optimized 
            cre-recombinase Expression Plasmids for use in Yeast.
  JOURNAL   Yeast. 2015 Feb 16. doi: 10.1002/yea.3069.
  PUBMED    25688547
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Feb 16. doi: 10.1002/yea.3069."
COMMENT     Sequence Label: pPL5071_TEF1*-Cre_URA3
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(5..252)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     218..236
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 ggccgcaaat taaagccttc gagcgtccca aaaccttctc aagcaaggtt ttcagtataa
       61 tgttacatgc gtacacgcgt ctgtacagaa aaaaaagaaa aatttgaaat ataaataacg
      121 ttcttaatac taacataact ataaaaaaat aaatagggac ctagacttca ggttgtctaa
      181 ctccttcctt ttcggttaga gcggatgtgg ggggagggcg tgaatgtaag cgtgacataa
      241 ctaattacat gactcgaggt cgacaattcc aaccttaccc aagagttcgc caaactcaga
      301 catcacttta gcaaaaccgc gccgtgcttc ttcctcggtg gcattcatca cgaaatgttc
      361 agcactacgc atacttttga caggaaacgc aacggatatt gagtcaatat caggcattct
      421 atcgctcagc tttacagtga caatgacggc tggcgactga atattagtgc ttacagacag
      481 cactacatat tttccgtcga tgttgaaatc ctttctcata tgtcaccata aatatcaaat
      541 aattatagca atcatttacg cgttaatggc taatcgccat cttccagcag gcgcaccatt
      601 gcccctgttt cactatccag gttacggata tagttcatga caatatttac attggtccag
      661 ccaccagctt gcatgatctc cggtattgaa actccagcgc gggccatatc tcgcgcggct
      721 ccgacacggg cactgtgtcc agaccaggcc aggtatctct gaccagagtc atccttagcg
      781 ccgtaaatca atcgatgagt tgcttcaaaa atcccttcca gggcgcgagt tgatagctgg
      841 ctggtggcag atggcgcggc aacaccattt tttctgaccc ggcaaaacag gtagttattc
      901 ggatcatcag ctacaccaga gacggaaatc catcgctcga ccagtttagt tacccccagg
      961 ctaagtgcct tctctacacc tgcggtgcta accagcgttt tcgttctgcc aatatggatt
     1021 aacattctcc caccgtcagt acgtgagata tctttaaccc tgatcctggc aatttcggc
//
