LOCUS       sequence-100320-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are 
            expressed as a single poly-cistronic unit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Chou BK, Mali P, Huang X, Ye Z, Dowey SN, Resar LM, Zou C, Zhang YA,
            Tong J, Cheng L
  TITLE     Efficient human iPS cell derivation by a non-integrating plasmid 
            from blood cells with unique epigenetic and gene expression 
            signatures.
  JOURNAL   Cell Res. 2011 Mar;21(3):518-29. doi: 10.1038/cr.2011.12. Epub 2011 
            Jan 18.
  PUBMED    21243013
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cr.2011.12"; journalName: "Cell Res"; date: "2011-03"; 
            volume: "21"; issue: "3"; pages: "518-29"
COMMENT     Sequence Label: pEB-C5
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(655..708)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ggatccggcc ttgccggcct cgagtcaatt ctgggcttct gggagcaggg cagggctgtg
       61 gatctcttcc tcttcctccc ggaagtaggc aggctttccc tgagaactgg ggccctgctg
      121 ggccttcagt ggacacgagg ccaccatatg gttgatgctt tggcaaaagt ggcacttctt
      181 gggctggggt ggcagcttgc attccttggc atgatggtct agcccaccgc agttgtagca
      241 cctgtctcct ttggatcttc gcttctgcat gttcttcccc tttggccgcc gctcacttcc
      301 aatacagaac acaccaccag ggccagtgac acggatggat tccagaccct tggcagactt
      361 cttaaaggtg aactccaccg cctcaccctc cttgaggctt cggaaccctt ccatgtgcag
      421 cttgctctgg tgcacaaaga cgtccaccgg ggggtcgagc gcgaccccgg cgcgggcggt
      481 catagacagg aagccgaacc ccatgcgcac gttgaaccac ttacagatgc cggccccgtg
      541 cagcagctgc ggctcgtctg ccgctcgggc cgcgtcaggc ggcgcctcct ctggcgcctt
      601 ctccgctgcc ttggcgcagc cacctgcaaa ctgctggttg gacaccgagc ccattgggcc
      661 aggattctcc tccacgtcac cgcatgttag aagacttcct ctgccctctc ctccggaccc
      721 gccgcctgca ccagagtttc gaagctgttc gagtttgtgt ttcaactgtt ctcgtcgttt
      781 cctcaataag tccttttcag aggtgagctt gtgctcgtct gcttgaatgg acaggatgta
      841 ggcggtggct tttttgagga tcactacctt gggggccttt tcgttgtttt ccaattcagg
      901 gatctggtca cgcagggcaa aaaagctgcg cttcagctcg ttcctcctct gacgttccaa
      961 gacgttgtgt gtccgcctct tgtcgttttc ctccgtgtct gaggacctgg gg
//
