LOCUS       sequence-100331-         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Characteristic fingerprint molecule fused to cohesin type III domain
            to perform single molecule force spectroscopy experiments; 
            covalently immobilized via introduced ybbR tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Schoeler C, Malinowska KH, Bernardi RC, Milles LF, Jobst MA, Durner 
            E, Ott W, Fried DB, Bayer EA, Schulten K, Gaub HE, Nash MA
  TITLE     Ultrastable cellulosome-adhesion complex tightens under load.
  JOURNAL   Nat Commun. 2014 Dec 8;5:5635. doi: 10.1038/ncomms6635.
  PUBMED    25482395
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-12-8"; volume: "5:5635. doi"; pages: " 
            10.1038/ncomms6635"
COMMENT     Sequence Label: pET28a-CohIII-CBM-HIS-ybbR
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgggtacc gcgctcacag acagaggaat
       61 gacttacgat ctcgatccta aggatggatc atctgctgct acaaagccag ttctcgaagt
      121 aactaagaag gttttcgaca cagctgctga tgctgctggc cagactgtta cagttgaatt
      181 caaggttagc ggtgcagaag gcaagtacgc tacaactggt tatcacatct actgggatga
      241 gagacttgaa gttgttgcaa ccaagacagg tgcatacgct aagaagggcg cagctcttga
      301 agattcaagc ttagctaagg ctgagaacaa cggtaacggt gttttcgttg cttcaggtgc
      361 tgatgatgat tttggtgctg acggtgtaat gtggactgtt gaactcaagg ttccagcaga
      421 cgctaaggct ggcgatgtat atccaatcga cgttgcttat cagtgggatc cttcaaaggg
      481 tgaccttttc acagataaca aggactcagc acagggcaag ctgatgcagg cttacttctt
      541 cacacagggt atcaagtcat cttctaatcc ttcaacagat gagtacctcg taaaggctaa
      601 cgctacatat gctgatggtt acattgcaat caaggctggt gagccaggca gtgtagtacc
      661 atcaacacag cctgtaacaa caccacctgc aacaacaaaa ccacctgcaa caacaatacc
      721 gccgtcagat gatccgaatg ccatggccaa t
//
