LOCUS       sequence-100397-        9229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation vector pBGRY.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Koh TW, He Z, Gorur-Shandilya S, Menuz K, Larter NK, Stewart S, 
            Carlson JR
  TITLE     The Drosophila IR20a clade of ionotropic receptors are candidate 
            taste and pheromone receptors.
  JOURNAL   Neuron. 2014 Aug 20;83(4):850-65. doi: 10.1016/j.neuron.2014.07.012.
            Epub 2014 Aug 7.
  PUBMED    25123314
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.07.012"; journalName: "Neuron"; date: 
            "2014-08-20- 20"; volume: "83"; issue: "4"; pages: "850-65"
COMMENT     Sequence Label: pBGRY
FEATURES             Location/Qualifiers
     source          1..9229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    76..506
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    549..673
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(698..703,704..721,722..728)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             782..1441
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(848..867)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1783..2088
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1967..1986
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2129..2253)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(join(3752..4366,4437..4568,4772..5087,5149..5803,
                     5878..6151,6553..6624))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     7076..7094
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    7319..7388
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(7475..8063)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7555..7574)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8234..9025,9026..9094))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8857..8876
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9095..9199)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttgt tggttggcac accacaaata tactgttgcc gagcacaatt
      121 gatcggctaa atggtatggc aagaaaaggt atgcaatata ataatctttt attgggtatg
      181 caacgaaaat ttgtttcgtc aacgtatgca atatttttta ttaaaagagg gtatgcaatg
      241 tattttatta aaaacgggta tgcaatataa taatctttta ttgggtatgc aacgaaaatt
      301 tgtttcgtca aagtatgcaa tattttttat taaaagaggg tatgcaatgt attttattaa
      361 aaacgggtat gcaataaaaa attatttggt ttctctaaaa agtatgcagc acttattttt
      421 tgataaggta tgcaacaaaa ttttactttg ccgaaaatat gcaatgtttt tgcgaataaa
      481 ttcaacgcac acttattacg tggccagttt aaacggccgg ccctttcgtc ttcaagaatt
      541 cgtttatcac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca
      601 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
      661 tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc
      721 gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa
      781 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
      841 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
      901 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
      961 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
     1021 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     1081 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     1141 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     1201 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     1261 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     1321 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     1381 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     1441 aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
     1501 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct
     1561 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
     1621 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
     1681 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
     1741 aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac
     1801 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     1861 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     1921 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     1981 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
     2041 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
     2101 cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta
     2161 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
     2221 cgtttctcgt tcagctttct tgtacaaagt ggtgataaac ggccggagta ctactaccag
     2281 gcaagaaagt aggttgataa agctatgcac aagatctgtc atatggatgg gtttcgcatc
     2341 ttctggatca taggacttga gaacatacac aaacacaact aaaatcagaa aaggttgcca
     2401 gcaawcactt tagttttctg caagctagat ccacctgcta ctatccctac tttcttctgt
     2461 tggggttgtc agagctctac ctacagttga ttaaaccaca catcagtcat cgtgtttttt
     2521 ttacaatttt cctaatttcc ttacttgtat tgcctacttt tttgcgaggg acttctattg
     2581 gccagtatat attatgtgtt gcatactctt ggtcacagca aaaataaaac aaaaaatacg
     2641 catggtaaaa gaatgcttcc ttttttacgc acaacggtag acgaagcata taatttttac
     2701 atatcggaaa tgttcttgaa acagaggtac cggatccccg cgcggccgct ggccacgggt
     2761 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg
     2821 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc
     2881 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac
     2941 gcggaagtca gcgccctgca ccattatgtt ccggaggcgc gccctagttc cagtgaaatc
     3001 caagcatttt ctaaattaaa tgtattctta ttattatagt tgttattttt gatatatata
     3061 aacaacacta ttatgcccac catttttttg agatgcatct acacaaggaa caaacactgg
     3121 atgtcacttt cagttcaaat tgtaacgcta atcactccga acaggtcaca aaaaattacc
     3181 ttaaaaagtc ataatattaa attagaataa atatagctgt gagggaaata tatacaaata
     3241 tattggagca aataaattgt acatacaaat atttattact aatttctatt gagacgaaat
     3301 gaaccactcg gaaccatttg agcgaaccga atcgcgcgga actaacgaca gtcgctccaa
     3361 ggtcgtcgaa caaaaggtga atgtgttgcg gagagcgggt gggagacagc gaaagagcaa
     3421 ctacgaaacg tggtgtggtg gaggtgaatt atgaagaggg cgcgcgattt gaaaagtatg
     3481 tatataaaaa atatatcccg gtgttttatg tagcgataaa cgagtttttg atgtaaggta
     3541 tgcaggtgtg taagtctttt ggttagaaga caaatccaaa gtctacttgt ggggatgttc
     3601 gaaggggaaa tacttgtatt ctataggtca tatcttgttt ttattggcac aaatataatt
     3661 acattagctt tttgaggggg caataaacag taaacacgat ggtaataatg gtaaaaaaaa
     3721 aaaacaagca gttatttcgg atatatgtcg gctactcctt gcgtcgggcc cgaagtctta
     3781 gagccagata tgcgagcacc cggaagctca cgatgagaat ggccagaccc acgtagtcca
     3841 gcggcagatc ggcggcggag aagttaagcg tctccaggat gaccttgccc gaactggggc
     3901 acgtggtgtt cgacgatgtg cagctaattt cgcccggctc cacgtccgcc cattggttaa
     3961 tcagcagacc ctcgttggcg taacggaacc atgagaggta cgacaaccat ttgaggtata
     4021 ctggcaccga gcccgagttc aagaagaagc cgccaaagag caggaatggt atgataaccg
     4081 gcggacccac agacagcgcc atcgaggtcg aggagctggc gcaggatatt agatatccga
     4141 aggacgttga cacattggcc accagagtga ccagcgccag gcagttgaag aagtgcagca
     4201 ctccggcccg cagtccgatc atcggatagg caatcgccgt gaagaccagt ggcactgtga
     4261 gaaaaagcgg caattcggca atcgttttgc ccagaaagta tgtgtcacag cgataaagtc
     4321 gacttcgggc ctccctcata aaaactggca gctctgaggt gaacacctaa atcgaatcga
     4381 ttcattagaa agttagtaaa ttattgaaat gcaaatgtat tctaaacatg acttacattt
     4441 atcgtggcaa agacgttttg aaaggtcatg ttggtcagga agaggaagat ggctccgttg
     4501 atattcatca cacccacttg cgtgagttgt tggcccaaaa agatgaggcc aatcaagatg
     4561 gcaaccatct gcaaattaaa atgttactcg catctcatta atattcgcga gttaaatgaa
     4621 atttatttat cttctgcaaa actataaact atacatctca ttgaaaaaaa ctaagaaggg
     4681 tgtggaatca ggcaattcta tctaaaatct agcgaatttg tttccaagaa ttgtaagcgt
     4741 tatatcattt gtttccactg gaaccactca ccgttgtctg aataagtcgc acttttacga
     4801 ggagtggttc cttgagcacc gacagccagg atcgccacag gaccgcccgg aactgcatga
     4861 accaggtggc cttgtaggtg tacccattct ccggctgctc cagtggcttc tccagatttt
     4921 tggtggccaa caactgctcc atatcccggg ctactttgct aatggcaaaa ttgtcgcata
     4981 tcttggcgat ccgatcacgg gactcgatct cccgtccggg cacaacggcc aacacctgta
     5041 cgtaaaagtc cgccggattg tagttggtag gacactgggc acccacgctg gataggagtt
     5101 gagatgttat gtaatactag atacccttaa taaacacatc gaactcacta ggaaaagaag
     5161 tcgacggctt cgctgggagt gcccaagaaa gctaccctgc cctcggccat cagaaggatc
     5221 ttgtcaaaga gctcaaacag ctcggaagac ggctgatgaa tggtcaggat gacggtcttg
     5281 cccttctgcg acagcttctt cagcacctgg acgacgctgt gggcggtaaa ggagtccagt
     5341 ccggaggtgg gctcatcgca gatcagaagc ggcggatcgg ttagagcctc ggaggcgaat
     5401 gccagacgct tcctttctcc gccggacaga cctttcaccc tgccgggcac accgatgatc
     5461 gtgtgctgac atttgctgag cgaaagctcc tggatcacct gatccacgcg ggccactcgc
     5521 tgccgatagg tcagatgtcg tggcatccgc accatggctt ggaaaatcag gtgttccctg
     5581 gccgttaggg agccgataaa gaggtcatcc tgctggacat aggcgcacct ggcctgcatc
     5641 tccttggcgt ccacaggttg gccattgagc agtcgcatcc cggatggcga tacttggatg
     5701 ccctgcggcg atcgaaaggc aagggcattc agcagggtcg tctttccggc accggaactg
     5761 cccatcacgg ccaaaagttc gcccggatag gccacgccgc aaactgagtt tcaaattggt
     5821 aattggaccc tttattaaga tttcacacag atcagccgac tgcgaataga aactcaccgt
     5881 tcttgagcaa atgtttcctg ggcgccggta tgtgtcgctc gttgcagaat agtccgcgtg
     5941 tccggttgac cagctgccgc catccggagc ccggctgatt gaccgcccca aagatgtcca
     6001 tattgtgcca ggcataggtg aggttctcgg ctagttggcc gctccctgaa ccggagtcct
     6061 ccggcggact gggtggccgg agcgtgccgt agtttttggc ctgcccgaag ccctggttaa
     6121 tgcagctctg cgaagccgct ccgctgtcac cctgcaatga taggggatct caaatatcaa
     6181 ctactagcgt tatgctcatc taaccccgaa caaaaagtac cccgaagtat cctacgaagt
     6241 aggtttatac ttttatttat tttttgtgca tctaggatca gcttaaaata tctggttgtt
     6301 atattttttg taaaaaagaa tatagtcgaa aatgaatgcc tttagatgtc ttgatcatga
     6361 tatgatctca aaaattgtct tatatagcga gaacagctac cagaataatc tgtttcgtgt
     6421 cactatttgt ttgtgcaatt gcggtttggg atttttgtgg gtcgcagttc tcacgccgca
     6481 tacaatttga tgttgcaatc gcagttccta tagatcaagt gaacttaaga tgtatgcaca
     6541 tgtactactc acattgttca gatgctcggc agatgggtgt ttgctgcctc cgcgaattaa
     6601 tagctcctga tcctcttggc ccattgccgg gatttttcac actttcccct gcttacccac
     6661 ccaaaaccaa tcaccacccc aatcactcaa aaaacaaaca aaaataagaa gcgagaggag
     6721 ttttggcaca gcactttgtg tttaattgat ggcgtaaacc gcttggagct tcgtcacgaa
     6781 accgctgaca aaatgcaact gaaggcggac attgacgcta cgtaacgcta caaacggtgg
     6841 cgaaagagat agcggacgca gcggcgaaag agacggcgat atttctgtgg acagagaagg
     6901 aggcaaacag cgctgacttt gagtggaatg tcattttgag tgagaggtaa tcgaaagaac
     6961 ctggtacatc aaataccctt ggatcgaagt aaatttaaaa ctgatcagat aagttcaatg
     7021 atatccagtg cagtaaaaaa aaaaaatgtt ttttttatct actttccgca aaaatgggtt
     7081 ttattaactt acatacatac taggcgcgcc catatgttcg gcttgtcgac atgcccgccg
     7141 tgaccgtcga gaacccgctg acgctgcccc gcgtatccgc acccgccgac gccgtcgcac
     7201 gtcccgtgct caccgtgacc accgcgccca gcggtttcga gggcgagggc ttcccggtgc
     7261 gccgcgcgtt cgccgggatc aactaccgcc acctcgaccc gttcatcatg atggaccaga
     7321 tgggtgaggt ggagtacgcg cccggggagc ccaagggcac gccctggcac ccgcaccgcg
     7381 gcttcgagac cgtgacctac atcgtcgacg gtaacatgtg agcaaaaggc cagcaaaagg
     7441 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7501 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7561 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7621 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7681 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7741 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7801 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7861 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7921 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7981 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8041 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8101 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8161 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8221 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8281 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8341 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8401 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8461 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8521 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8581 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8641 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8701 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8761 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8821 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8881 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8941 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9001 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9061 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9121 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9181 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgagtc
//
