LOCUS       sequence-100400-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A fluorescent reporter of AMPK activity and cellular energy stress.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Tsou P, Zheng B, Hsu CH, Sasaki AT, Cantley LC
  TITLE     A fluorescent reporter of AMPK activity and cellular energy stress.
  JOURNAL   Cell Metab. 2011 Apr 6;13(4):476-86.
  PUBMED    21459332
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Metab."; date: "2011-04-6"; volume: "13(4)"; pages: "476-86"
COMMENT     Sequence Label: ampkar
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..20
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2..19
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(84..818)
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     277..296
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     536..557
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(663..684)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 catttaggtg acactataga atagggccct ctagatgcat gctcgagcgg ccgccagtgt
       61 gatggatatc tgcagaattc ttactcgatg ttgtggcgga tcttgaagtt ggccttgatg
      121 ccgttcttct gcttgtcggc ggtgatatag acgttgtggc tgttgtagtt gtactccagc
      181 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc
      241 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg
      301 aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc
      361 tgcttcatgt ggtcggggta gcgggcgaag cactgaaggc cgtagcccag ggtggtcacg
      421 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tcagcttcag ggtcagcttg
      481 ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg
      541 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc
      601 atagatccac cggtacctcc cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
      661 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagcttg
      721 gactggtagc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
      781 ttctgctggt agtggtcggc gagctgcacg ccgcccatga gctcgctgcc gccggtgccg
      841 aggtccacca gggtggcgac acgacgcatc ttggtgctgc cctcgccgct gcctggctta
      901 cctgcagagc gatcaacttt gttctgctcg aggcactgct taaatttgtc gtttatgaaa
      961 atgaccagag ataaaatatc tgattccacg cctacaccaa cggttatttc atcaccttga
     1021 gacag
//
