LOCUS       sequence-100430-         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Diacylglycerol (DAG) reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Tsai FC, Seki A, Yang HW, Hayer A, Carrasco S, Malmersjo S, Meyer T
  TITLE     A polarized Ca(2+), diacylglycerol and STIM1 signalling system 
            regulates directed cell migration.
  JOURNAL   Nat Cell Biol. 2014 Feb;16(2):133-44. doi: 10.1038/ncb2906. Epub 
            2014 Jan 26.
  PUBMED    24463606
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2906"; 
            journalName: "Nat Cell Biol"; date: "2014-02"; volume: "16"; issue: 
            "2"; pages: "133-44"
COMMENT     Sequence Label: C1A-C1A-EYFP
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(129..380)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(171..192)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     520..539
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tagagtcgcg
      121 gccgcttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
      181 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctggtagctc
      241 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
      301 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg
      361 atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc
      421 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
      481 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
      541 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
      601 tgctgcttca tgtggtcggg gtagcgggcg aagcactgca cgccgtagcc gaaggtggtc
      661 acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc
      721 ttgccgta
//
