LOCUS       sequence-100432-       12310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under 
            MtBCP1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12310)
  AUTHORS   Ivanov S, Harrison MJ
  TITLE     A set of fluorescent protein-based markers expressed from 
            constitutive and arbuscular mycorrhiza-inducible promoters to label 
            organelles, membranes and cytoskeletal elements in Medicago 
            truncatula.
  JOURNAL   Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706.
  PUBMED    25329881
REFERENCE   2  (bases 1 to 12310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 20. doi: 10.1111/tpj.12706."
COMMENT     Sequence Label: pCMB-NUCr
FEATURES             Location/Qualifiers
     source          1..12310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    239..263
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(356..608)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(648..1442)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(759..778)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1369..1388
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1476..1655)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1773..1795
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1792..1808
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1792..1808
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1828..1848
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3030..3054
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3105..3812
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3518..3537
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3813..5624
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     protein_bind    complement(5626..5650)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5881..5901)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(5909..5926)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5909..5925)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5918..5940)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6014..6038
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7338..7967
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8401..9468
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      9534..9728
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9964..9986)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10072..10212
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10127..10146
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10227..10244)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10398..10986)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10478..10497)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11230..12021)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
      361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca gaagaactcg
      661 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
      721 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
      781 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
      841 ccattttcca ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg
      901 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
      961 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     1021 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     1081 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     1141 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     1201 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     1261 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     1321 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     1381 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     1441 atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1501 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1561 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     1621 cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     1681 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctcatg tatgataatt
     1741 cgagggtacc cggggatcct ctagagggcc cgagcggata acaatttcac acaggaaaca
     1801 gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttgga gagagggaga
     1861 tgtgttttta aggagctata gaaggataca ataccgcttc tttgattggt attctaactc
     1921 aatgcgttgc ttaagagttg gttaatctat ttgaaatgtt gcgattatag tagatcttgt
     1981 gctcccatct ttaatatatc ttttacactt actccttagt tatacaaaca ttaaacatca
     2041 taacttctaa ggtaattgat acgtgccaaa ttttctaaac tccattaaat ttggtttgaa
     2101 agaaatttca tgaaattcct ctagttcgtt ataaatatta gataattagg ttttgagtag
     2161 cattgtagct accatgacct atttccatta catttgtagg tttaaaagat caaagatgac
     2221 ttggggtatc aattaacgag tgagcatata tgcgagcaag gaaagagata agtcagagtg
     2281 tttaaggaaa gcaaggcaag aaaattcact tagggagcca aaacaaggtc aatactcgat
     2341 tacatctcta atttaagctt gagaacttga tatattcgag actcctcaag tatttgtact
     2401 ccaagtgtca atactcgatt acatctctaa ttttccaaat aaagaaactc gtaagaaaca
     2461 aacattattt atgtgtaaac tttctgcatg gagacaactc aattataacg aagcccggaa
     2521 gaggaactat atatactgtt tctatttccc tttgacacgg tccacactat attgcatact
     2581 tgtttactgc caaataaaaa caagtatgtc cacgtggtta aataaaaaca aaagaaaact
     2641 ttctttttgt tcaagtgata gaaagtttct ttacattaat ataaaataat ataagtgtat
     2701 tccaaaaagg taaaaaaaaa aataataagt tgtggaaatt caacctcgtc ttagtaaaaa
     2761 gccgaccaaa tccggtagcc gtttataata gcaaacaaat attctattat tgttgtgacg
     2821 ataaaatcaa tacacccaca catgacaact atatatagcc taagttgata tgcacaattc
     2881 atcaaatcaa atcaaaacaa ttacatttgc taacttgcta ttcctttgat tgagatttta
     2941 ttctaggaaa taaaattttg acactcattt ttttaaagtt aatttctaca tttagagaga
     3001 aatattttcc aaaatcaaat tttgatcaag caagtttgta caaaaaagca ggcttcatgc
     3061 agccttctct taaacgcatg aaaatagagc catcttctca acctatggtg agcaagggcg
     3121 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     3181 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     3241 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     3301 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     3361 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     3421 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     3481 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     3541 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     3601 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     3661 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     3721 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     3781 actccaccgg cggcatggac gagctgtaca agatgttacg tcctgtagaa accccaaccc
     3841 gtgaaatcaa aaaactcgac ggcctgtggg cattcagtct ggatcgcgaa aactgtggaa
     3901 ttgatcagcg ttggtgggaa agcgcgttac aagaaagccg ggcaattgct gtgccaggca
     3961 gttttaacga tcagttcgcc gatgcagata ttcgtaatta tgcgggcaac gtctggtatc
     4021 agcgcgaagt ctttataccg aaaggttggg caggccagcg tatcgtgctg cgtttcgatg
     4081 cggtcactca ttacggcaaa gtgtgggtca ataatcagga agtgatggag catcagggcg
     4141 gctatacgcc atttgaagcc gatgtcacgc cgtatgttat tgccgggaaa agtgtacgta
     4201 tcaccgtttg tgtgaacaac gaactgaact ggcagactat cccgccggga atggtgatta
     4261 ccgacgaaaa cggcaagaaa aagcagtctt acttccatga tttctttaac tatgccggaa
     4321 tccatcgcag cgtaatgctc tacaccacgc cgaacacctg ggtggacgat atcaccgtgg
     4381 tgacgcatgt cgcgcaagac tgtaaccacg cgtctgttga ctggcaggtg gtggccaatg
     4441 gtgatgtcag cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact ggacaaggca
     4501 ctagcgggac tttgcaagtg gtgaatccgc acctctggca accgggtgaa ggttatctct
     4561 atgaactgtg cgtcacagcc aaaagccaga cagagtgtga tatctacccg cttcgcgtcg
     4621 gcatccggtc agtggcagtg aagggcgaac agttcctgat taaccacaaa ccgttctact
     4681 ttactggctt tggtcgtcat gaagatgcgg acttgcgtgg caaaggattc gataacgtgc
     4741 tgatggtgca cgaccacgca ttaatggact ggattggggc caactcctac cgtacctcgc
     4801 attaccctta cgctgaagag atgctcgact gggcagatga acatggcatc gtggtgattg
     4861 atgaaactgc tgctgtcggc tttaacctct ctttaggcat tggtttcgaa gcgggcaaca
     4921 agccgaaaga actgtacagc gaagaggcag tcaacgggga aactcagcaa gcgcacttac
     4981 aggcgattaa agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg atgtggagta
     5041 ttgccaacga accggatacc cgtccgcaag gtgcacggga atatttcgcg ccactggcgg
     5101 aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta atgttctgcg
     5161 acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac cgttattacg
     5221 gatggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa aaagaacttc
     5281 tggcctggca ggagaaactg catcagccga ttatcatcac cgaatacggc gtggatacgt
     5341 tagccgggct gcactcaatg tacaccgaca tgtggagtga agagtatcag tgtgcatggc
     5401 tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa caggtatgga
     5461 atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac aagaaaggga
     5521 tcttcactcg cgaccgcaaa ccgaagtcgg cggcttttct gctgcaaaaa cgctggactg
     5581 gcatgaactt cggtgaaaaa ccgcagcagg gaggcaaaca atgagaccca gctttcttgt
     5641 acaaagtgga ataagcggcc atgctagagt ccgcaaaaat caccagtctc tctctacaaa
     5701 tctatctctc tctatttttc tccagaataa tgtgtgagta gttcccagat aagggaatta
     5761 gggttcttat agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg
     5821 tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtgacg
     5881 caactttatt atacatagtt gataattcac tggccgtcgt tttacaacgt cgtgactggg
     5941 aaaacgagct caagcttagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta
     6001 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     6061 agaataacgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
     6121 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
     6181 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
     6241 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
     6301 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
     6361 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
     6421 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
     6481 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
     6541 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
     6601 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
     6661 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
     6721 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
     6781 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
     6841 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
     6901 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
     6961 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
     7021 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
     7081 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
     7141 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
     7201 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
     7261 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
     7321 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
     7381 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
     7441 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
     7501 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
     7561 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
     7621 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
     7681 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
     7741 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
     7801 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
     7861 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
     7921 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
     7981 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
     8041 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
     8101 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
     8161 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
     8221 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
     8281 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
     8341 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgac
     8401 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     8461 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     8521 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     8581 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     8641 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     8701 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     8761 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     8821 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     8881 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     8941 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     9001 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     9061 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     9121 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     9181 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     9241 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     9301 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     9361 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     9421 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     9481 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat
     9541 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     9601 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
     9661 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
     9721 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
     9781 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
     9841 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
     9901 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
     9961 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    10021 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
    10081 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
    10141 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
    10201 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    10261 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    10321 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    10381 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    10441 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    10501 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    10561 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    10621 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    10681 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    10741 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    10801 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
    10861 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    10921 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    10981 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    11041 cgttaaggga ttttggtcat gcatgatata tctcccaatt tgtgtagggc ttattatgca
    11101 cgcttaaaaa taataaaagc agacttgacc tgatagtttg gctgtgagca attatgtgct
    11161 tagtgcatct aatcgcttga gttaacgccg gcgaagcggc gtcggcttga acgaatttct
    11221 agctagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc
    11281 ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct
    11341 agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc
    11401 gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag
    11461 cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc
    11521 atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg
    11581 acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc
    11641 gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg
    11701 cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac
    11761 ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt
    11821 gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat
    11881 atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa
    11941 cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc
    12001 ggcgatcacc gcttccccca tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt
    12061 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
    12121 ggatgcccga ggcatagact gtaccccaaa aaaacatgtc ataacaagaa gccatgaaaa
    12181 ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgacg
    12241 gcagttacgc tacttgcatt acagcttacg aaccgaacga ggcttatgtc cactgggttc
    12301 gtgcccgaat
//
