LOCUS       sequence-100579-       11345 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for mitochondria ScCOX4sp-mCherry expressed 
            under AtUBQ10 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11345)
  AUTHORS   Ivanov S, Harrison MJ
  TITLE     A set of fluorescent protein-based markers expressed from 
            constitutive and arbuscular mycorrhiza-inducible promoters to label 
            organelles, membranes and cytoskeletal elements in Medicago 
            truncatula.
  JOURNAL   Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706.
  PUBMED    25329881
REFERENCE   2  (bases 1 to 11345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 20. doi: 10.1111/tpj.12706."
COMMENT     Sequence Label: pCMU-MITr
FEATURES             Location/Qualifiers
     source          1..11345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    239..263
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(356..608)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(648..1442)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(759..778)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1369..1388
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1476..1655)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1773..1795
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1792..1808
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1792..1808
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1828..1848
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3835..3859
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3862..3927
                     /codon_start=1
                     /product="mitochondrial presequence of yeast cytochrome c 
                     oxidase subunit IV (Hurt et al., 1984)"
                     /label=pre-CoxIV
                     /translation="MLSLRQSIRFFKPATRTLCSSR"
     CDS             3949..4659
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     4362..4381
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(4661..4685)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4916..4936)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4944..4961)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4944..4960)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4953..4975)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5049..5073
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6373..7002
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7436..8503
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8569..8763
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8999..9021)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9107..9247
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9162..9181
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9262..9279)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9433..10021)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9513..9532)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10265..11056)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
      361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca gaagaactcg
      661 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
      721 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
      781 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
      841 ccattttcca ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg
      901 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
      961 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     1021 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     1081 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     1141 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     1201 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     1261 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     1321 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     1381 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     1441 atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1501 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1561 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     1621 cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     1681 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctcatg tatgataatt
     1741 cgagggtacc cggggatcct ctagagggcc cgagcggata acaatttcac acaggaaaca
     1801 gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttgag tctagctcaa
     1861 cagagctttt aacccaaatt ggtacaatag aatacaactt tagatcataa ttctcaaaag
     1921 aaagagattc cttagctatt ctatctgcca ctccatttcc ttctcggctt gtatgcacaa
     1981 gcataaaatc ctcaaacttg ctaagtagat actttatgtc ttggataatt ggattgagac
     2041 ttgacaagca taactttcat gtaaccaaag acacaagttg ctgagaatcc acctcaaaaa
     2101 tgatcttcct ataattgaat cgggataatg acagcacagc ccatctaaga gcctccactt
     2161 ctacttccag cacgcttctt acttttacca cagctcttgc acctaaccat aacaccttcc
     2221 ctgtatgatc gcgaagcacc caccctaagc cacattttaa tccttctgtt ggccatgccc
     2281 catcaaagtt gcacttaacc caagattgtg gtggagcttc ccatgtttct cgtctgtccc
     2341 gacggtgttg tggttggtgc tttccttaca ttctgagcct ctttccttct aatccactca
     2401 tctgcatctt cttgtgtcct tactaatacc tcattggttc caaattccct ccctttaagc
     2461 accagctcgt ttctgttctt ccacagcctc ccaagtatcc aagggactaa agcctccaca
     2521 ttcttcagat caggatattc ttgtttaaga tgttgaactc tatggaggtt tgtatgaact
     2581 gatgatctag gaccggataa gttcccttct tcatagcgaa cttattcaaa gaatgttttg
     2641 tgtatcattc ttgttacatt gttattaatg aaaaaatatt attggtcatt ggactgaaca
     2701 cgagtgttaa atatggacca ggccccaaat aagatccatt gatatatgaa ttaaataaca
     2761 agaataaatc gagtcaccaa accacttgcc ttttttaacg agacttgttc accaacttga
     2821 tacaaaagtc attatcctat gcaaatcaat aatcatacaa aaatatccaa taacactaaa
     2881 aaattaaaag aaatggataa tttcacaata tgttatacga taaagaagtt acttttccaa
     2941 gaaattcact gattttataa gcccacttgc attagataaa tggcaaaaaa aaacaaaaag
     3001 gaaaagaaat aaagcacgaa gaattctaga aaatacgaaa tacgcttcaa tgcagtggga
     3061 cccacggttc aattattgcc aattttcagc tccaccgtat atttaaaaaa taaaacgata
     3121 atgctaaaaa aatataaatc gtaacgatcg ttaaatctca acggctggat cttatgacga
     3181 ccgttagaaa ttgtggttgt cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc
     3241 ggcacacacg agtcgtgttt atcaactcaa agcacaaata cttttcctca acctaaaaat
     3301 aaggcaatta gccaaaaaca actttgcgtg taaacaacgc tcaatacacg tgtcatttta
     3361 ttattagcta ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct cgtcttttct
     3421 tcttcttctt ctataaaaca atacccaaag agctcttctt cttcacaatt cagatttcaa
     3481 tttctcaaaa tcttaaaaac tttctctcaa ttctctctac cgtgatcaag gtaaatttct
     3541 gtgttcctta ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat
     3601 gttctttggt ttagattctg ttaatcttag atcgaagacg attttctggg tttgatcgtt
     3661 agatatcatc ttaattctcg attagggttt catagatatc atccgatttg ttcaaataat
     3721 ttgagttttg tcgaataatt actcttcgat ttgtgatttc tatctagatc tggtgttagt
     3781 ttctagtttg tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta acaggcaagt
     3841 ttgtacaaaa aagcaggctt catgctttca ctacgtcaat ctataagatt tttcaagcca
     3901 gccacaagaa ctttgtgtag ctctagatat ctgcttcagc aaaaacccat ggtgagcaag
     3961 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     4021 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     4081 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     4141 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     4201 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     4261 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     4321 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     4381 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     4441 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     4501 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     4561 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     4621 cgccactcca ccggcggcat ggacgagctg tacaagtaag acccagcttt cttgtacaaa
     4681 gtggaataag cggccatgct agagtccgca aaaatcacca gtctctctct acaaatctat
     4741 ctctctctat ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt
     4801 cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt
     4861 gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacgcaact
     4921 ttattataca tagttgataa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     4981 gagctcaagc ttagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta
     5041 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     5101 aacggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc
     5161 aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg
     5221 cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct
     5281 aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta
     5341 gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg
     5401 ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc
     5461 aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca
     5521 ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg
     5581 tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg
     5641 agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca
     5701 ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt
     5761 ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt
     5821 ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc
     5881 aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt
     5941 accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct
     6001 tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag
     6061 aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga
     6121 gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac
     6181 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc
     6241 cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
     6301 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
     6361 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
     6421 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
     6481 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
     6541 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
     6601 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
     6661 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
     6721 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
     6781 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
     6841 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
     6901 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
     6961 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
     7021 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
     7081 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
     7141 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
     7201 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
     7261 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
     7321 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
     7381 gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgacggtcg
     7441 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
     7501 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
     7561 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
     7621 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
     7681 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
     7741 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
     7801 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
     7861 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
     7921 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
     7981 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
     8041 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
     8101 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
     8161 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
     8221 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
     8281 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
     8341 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
     8401 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
     8461 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
     8521 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac
     8581 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
     8641 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
     8701 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
     8761 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
     8821 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
     8881 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
     8941 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     9001 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     9061 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     9121 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     9181 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     9241 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     9301 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     9361 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     9421 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9481 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9541 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9601 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     9661 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9721 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9781 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9841 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     9901 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9961 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    10021 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    10081 agggattttg gtcatgcatg atatatctcc caatttgtgt agggcttatt atgcacgctt
    10141 aaaaataata aaagcagact tgacctgata gtttggctgt gagcaattat gtgcttagtg
    10201 catctaatcg cttgagttaa cgccggcgaa gcggcgtcgg cttgaacgaa tttctagcta
    10261 gacattattt gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca
    10321 actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt
    10381 caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat
    10441 ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta
    10501 catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta
    10561 gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta
    10621 ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg
    10681 tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt
    10741 cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta
    10801 cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca
    10861 aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac
    10921 tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg
    10981 gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga
    11041 tcaccgcttc ccccatgatg tttaactttg ttttagggcg actgccctgc tgcgtaacat
    11101 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg
    11161 cccgaggcat agactgtacc ccaaaaaaac atgtcataac aagaagccat gaaaaccgcc
    11221 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgacggcagt
    11281 tacgctactt gcattacagc ttacgaaccg aacgaggctt atgtccactg ggttcgtgcc
    11341 cgaat
//
