LOCUS       sequence-100630-        1917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inducible dual expression of NS3 protease along with its cofactor 
            NS4A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Lee H, Zhu T, Patel K, Zhang YY, Truong L, Hevener KE, Gatuz JL, 
            Subramanya G, Jeong HY, Uprichard SL, Johnson ME
  TITLE     High-throughput screening (HTS) and hit validation to identify small
            molecule inhibitors with activity against NS3/4A proteases from 
            multiple hepatitis C virus genotypes.
  JOURNAL   PLoS One. 2013 Oct 9;8(10):e75144. doi: 
            10.1371/journal.pone.0075144. eCollection 2013.
  PUBMED    24130685
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-9"; volume: "8(10):e75144. doi"; pages: " 
            10.1371/journal.pone.0075144. eCollection 2013"
COMMENT     Sequence Label: pETDuet-1 Hepatitis C Virus NS3/NS4A
FEATURES             Location/Qualifiers
     source          1..1917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcaccacc atcatcacca tgcaccgatt accgcgtatt ctcagcaaac gcgcggtctg
       61 ctgggctgta ttatcacgtc cctgactggt cgtgacaaaa accaggtgga aggcgaagtg
      121 caggtcgttt ctactgcaac ccagtccttc ctggccacct gcgtaaacgg cgtctgctgg
      181 accgtttacc acggtgcggg cagcaaaacc ctggccgcgc cgaaaggccc aattactcag
      241 atgtatacca acgtagatca ggatctggtt ggttggccga aaccgcctgg tgcacgctcc
      301 ctgaccccgt gtacctgcgg ctcttccgac ctgtatctgg tcacgcgtca tgcggatgta
      361 atcccggttc gccgtcgcgg tgactcccgt ggtagcctgc tgtctccgcg tcctgttagc
      421 tacctgaaag gcagctccgg cggtccgctg ctgtgcccgt ttggccacgc agttggtatc
      481 ttccgtgcag ccgtttgcac gcgtggcgtt gccaaagccg ttgatttcgt tccggtggaa
      541 tctatggaaa ccaccatgcg ctctcctgtg tttaccgata acagctcccc accggcagtg
      601 ccgcaatctt tccaggttgc gcacctgcac gcaccaaccg gcagcggtaa aagcaccaaa
      661 gtgccggcgg cctacgcggc ccagggttac aaagtactgg ttctgaatcc gtccgttgca
      721 gcgacgctgg gtttcggtgc gtatatgtcc aaagctcacg gcatcgaccc taacatccgc
      781 actggtgtgc gcaccattac caccggcgca ccggtgacct attccaccta cggcaaattc
      841 ctggcggatg gtggttgctc cggtggcgca tacgatatca tcatttgcga tgagtgtcac
      901 tctactgaca gcactaccat cctgggtatt ggcaccgtgc tggatcaggc ggaaacggct
      961 ggtgctcgtc tggtggtact ggctaccgcg actccgccgg gtagcgttac cgtaccgcac
     1021 ccgaacatcg aagaggttgc gctgtctaat accggcgaaa tcccgttcta tggcaaggcc
     1081 atcccgattg aagccattcg cggcggtcgc catctgatct tctgtcactc caagaaaaaa
     1141 tgtgacgaac tggcggctaa actgagcggt ctgggtatca acgcagtggc gtactaccgt
     1201 ggtctggacg tgtctgttat tccgaccatc ggtgacgtcg tcgtagttgc tacggacgca
     1261 ctgatgaccg gctacaccgg tgacttcgac tctgtgatcg atagcaacac ctgcgtaacc
     1321 cagactgtgg atttctctct ggacccgacc tttactatcg aaaccactac tgtgccgcag
     1381 gacgctgtta gccgctctca acgtcgtggc cgtaccggtc gcggccgtcg tggcatttac
     1441 cgttttgtca ctccaggtga acgtccgagc ggcatgttcg actcctctgt tctgtgcgag
     1501 tgctacgacg ctggctgcgc gtggtacgaa ctgactcctg ctgagactag cgttcgtctg
     1561 cgtgcctacc tgaacacccc gggtctgcca gtgtgtcagg accacctgga attctgggaa
     1621 tccgttttta ccggcctgac tcacatcgat gcgcacttcc tgtctcagac taaacaagct
     1681 ggcgataact ttccgtacct ggttgcatat caggctactg tatgtgctcg tgcgcaggca
     1741 ccaccgccgt cctgggacca gatgtggaaa tgtctgatcc gtctgaaacc gaccctgcac
     1801 ggcccgaccc cgctgctgta tcgtctgggt gctgtccaga acgaggttac tctgacgcat
     1861 cctatcacca agtacatcat ggcttgcatg agcgctgacc tggaggttgt tacttaa
//
