LOCUS       sequence-100662-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration-free (episomal) expression of human OCT3/4 and shRNA 
            against p53.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 May;8(5):409-12
  PUBMED    21460823
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-05"; volume: "8(5)"; pages: "409-1"
COMMENT     Sequence Label: pCXLE-hOCT3/4-shp53-F
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        201..514
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     433..453
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(665..682)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(916..935)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taaggatgtc aactacagtc agagaacccc tttgtgtttg gtcccccccc gtgtcacatg
       61 tggaacaggg cccagttggc aagttgtacc aaccaactga agggattaca tgcactgccc
      121 cgcgaagaag gggcagagat gtcgtagtca ggtttagttc gtccggggcg gggatcgatc
      181 ctctagagtc gacgctagcg gatccgacgc cgccatctct aggcccgcgc cggccccctc
      241 gcacagactt gtgggagaag ctcggctact cccctgcccc ggttaatttg catataatat
      301 ttcctagtaa ctatagaggc ttaatgtgcg ataaaagaca gataatctgt tctttttaat
      361 actagctaca ttttacatga taggcttgga tttctataag agatacaaat actaaattat
      421 tattttaaaa aacagcacaa aaggaaactc accctaactg taaagtaatt gtgtgttttg
      481 agactataaa tatcccttgg agaaaagcct tgtttgggcc gactccagtg gtaatctact
      541 tcaagagagt agattaccac tggagtcttt tttggaattc ctgcagcccg ggggatccgc
      601 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
      661 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      721 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      781 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      841 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      901 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      961 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1021 cgctttctca tagctcacgc tgtaggtatc tcagttcggt g
//
