LOCUS       sequence-100667-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a guide RNA (gRNA) to target human IRF-1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Fujita T, Fujii H
  TITLE     Efficient isolation of specific genomic regions and identification 
            of associated proteins by engineered DNA-binding molecule-mediated 
            chromatin immunoprecipitation (enChIP) using CRISPR.
  JOURNAL   Biochem Biophys Res Commun. 2013 Sep 13;439(1):132-6. doi: 
            10.1016/j.bbrc.2013.08.013. Epub 2013 Aug 11.
  PUBMED    23942116
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2013.08.013"; journalName: "Biochem Biophys Res 
            Commun"; date: "2013-09-13- 13"; volume: "439"; issue: "1"; pages: 
            "132-6"
COMMENT     Sequence Label: gRNA-hIRF-1 #12
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    102..126
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(138..213)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(234..482)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(292..311)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(462..482)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(621..640)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(622..640)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(647..664)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(647..663)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(656..678)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     982..1001
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
       61 ctagtaacgg ccgccagtgt gctggaattc gccctttaat gccaactttg tacaagaaag
      121 ctgggtctag aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc
      181 cttattttaa cttgctattt ctagctctaa aacggacagc ccagcgcccc cgcggtgttt
      241 cgtcctttcc acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc
      301 atatgatagt ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac
      361 tttctacgtc acgtattttg tactaatatc tttgtgttta cagtcaaatt aattctaatt
      421 atctctctaa cagccttgta tcgtatatgc aaatatgaag gaatcatggg aaataggccc
      481 tcttcctgcc cgaccttggt accggatcca gtcgactgaa ttggttcctt taaagcctgc
      541 ttttttgtac aaagggcgaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg
      601 catctagagg gcccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
      661 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      721 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      781 tgcgcagcct atacgtacgg cagtttaagg tttacaccta taaaagagag agccgttatc
      841 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc ggggcgacgg atggtgatcc
      901 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
      961 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     1021 ttatcgggga agaagtggct
//
