LOCUS       sequence-100680-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of BFP, rtTA and 
            hygromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-BH
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             588..698
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             711..767
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tggaattact caatggagtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc
       61 tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg
      121 ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt
      181 catggcaaga ctttctgcgg aacaacgcca agtcattccg ctgtgctctc ctctcacatc
      241 gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg
      301 aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc
      361 tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag
      421 caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa
      481 ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca
      541 tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccggccgac gcccttgacg
      601 attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc
      661 ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtccggc gctactaact
      721 tcagcctgct gaagcaggct ggtgacgtcg aggagaatcc tggtcccatg aaaaagcctg
      781 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
      841 tgatgcagct ctcgga
//
