LOCUS       sequence-100681-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of BFP, rtTA and 
            hygromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-BH
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             236..292
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacatca agaccacata
       61 tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag
      121 actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt
      181 ggccagatac tgcgacctcc ctagcaaact ggggcacaag ctgaacggca gtggagctac
      241 taacttcagc ctgctgaagc aggctggtga cgtcgaggag aatcctggcc ccatgtctag
      301 actggacaag agcaaagtca taaacggcgc tctggaatta ctcaatggag tcggtatcga
      361 aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg
      421 gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca
      481 tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc
      541 caagtcattc cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac
      601 ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca
      661 aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg
      721 ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac
      781 cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc agggagccga
      841 acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg
      901 cgaaa
//
