LOCUS       sequence-100685-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of RFP, rtTA and 
            puromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-RP
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..98
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(99..716,717..722,723..758,759..761,762..797,798..800,
                     801..836,837..842)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 tgttcctgta cggcatggac gagctgtaca agggcagtgg agctactaac ttcagcctgc
       61 tgaagcaggc tggtgacgtc gaggagaatc ctggccccat gtctagactg gacaagagca
      121 aagtcataaa cggcgctctg gaattactca atggagtcgg tatcgaaggc ctgacgacaa
      181 ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca
      241 agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct
      301 gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag tcattccgct
      361 gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga
      421 aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg
      481 agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg
      541 aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc
      601 cacttctgag acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt
      661 tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc
      721 cggccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact
      781 ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg
      841 ggtccggcgc tactaacttc agcctgctga agcaggc
//
